BD PharmingenTM Violet Live Cell Caspase Probe

Description

Two-color flow cytometric analysis of Viability and Caspase Activity in Camptothecin-treated Jurkat Cells. Cells from the human Jurkat Acute T cell leukemia, ATCC TIB-152 cell line were treated with 0.025 % DMSO Left Panel or 5 muM Camptothecin Right Panel for 16 hours. The cultured cells were then stained with BD Pharmingen Violet Live Cell Caspase Probe Cat. No. 565521 according to the recommended assay procedure. After recovery in media, cells were resuspended in DPBS and stained with BD Horizon Fixable Viability Stain 780 FVS780 , Cat. No. 565388 . Two-color flow cytometric contour plots showing the correlated expression of Violet Live Caspase Probe fluorescence versus BD Horizon Fixable Viability Stain 780 fluorescence were derived from gated events with the forward and side light-scatter characteristics of viable cells. Live cells are double negative for both fluorescent probes, whereas apoptotic cells are positive for Violet Live Cell Caspase Probe and negative for FVS780, and dead cells are positive for FVS780. Note that dead cells bind intermediate amounts of Violet Live Cell Caspase Probe. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Violet Live Cell Caspase Probe has been tested on mouse data not shown . Two-color flow cytometric analysis of Viability and Caspase Activity in Camptothecin-treated Jurkat Cells. Cells from the human Jurkat Acute T cell leukemia, ATCC TIB-152 cell line were treated with 0.025 % DMSO Left Panel or 5 muM Camptothecin Right Panel for 16 hours. The cultured cells were then stained with BD Pharmingen Violet Live Cell Caspase Probe Cat. No. 565521 according to the recommended assay procedure. After recovery in media, cells were resuspended in DPBS and stained with BD Horizon Fixable Viability Stain 780 FVS780 , Cat. No. 565388 . Two-color flow cytometric contour plots showing the correlated expression of Violet Live Caspase Probe fluorescence versus BD Horizon Fixable Viability Stain 780 fluorescence were derived from gated events with the forward and side light-scatter characteristics of viable cells. Live cells are double negative for both fluorescent probes, whereas apoptotic cells are positive for Violet Live Cell Caspase Probe and negative for FVS780, and dead cells are positive for FVS780. Note that dead cells bind intermediate amounts of Violet Live Cell Caspase Probe. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Violet Live Cell Caspase Probe has been tested on mouse data not shown . Two-color flow cytometric analysis of Viability and Caspase Activity in Camptothecin-treated Jurkat Cells. Cells from the human Jurkat Acute T cell leukemia, ATCC TIB-152 cell line were treated with 0.025 % DMSO Left Panel or 5 muM Camptothecin Right Panel for 16 hours. The cultured cells were then stained with BD Pharmingen Violet Live Cell Caspase Probe Cat. No. 565521 according to the recommended assay procedure. After recovery in media, cells were resuspended in DPBS and stained with BD Horizon Fixable Viability Stain 780 FVS780 , Cat. No. 565388 . Two-color flow cytometric contour plots showing the correlated expression of Violet Live Caspase Probe fluorescence versus BD Horizon Fixable Viability Stain 780 fluorescence were derived from gated events with the forward and side light-scatter characteristics of viable cells. Live cells are double negative for both fluorescent probes, whereas apoptotic cells are positive for Violet Live Cell Caspase Probe and negative for FVS780, and dead cells are positive for FVS780. Note that dead cells bind intermediate amounts of Violet Live Cell Caspase Probe. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Violet Live Cell Caspase Probe has been tested on mouse data not shown . Show More Two-color flow cytometric analysis of Viability and Caspase Activity in Camptothecin-treated Jurkat Cells. Cells from the human Jurkat Acute T cell leukemia, ATCC TIB-152 cell line were treated with 0.025 % DMSO Left Panel or 5 muM Camptothecin Right Panel for 16 hours. The cultured cells were then stained with BD Pharmingen Violet Live Cell Caspase Probe Cat. No. 565521 according to the recommended assay procedure. After recovery in media, cells were resuspended in DPBS and stained with BD Horizon Fixable Viability Stain 780 FVS780 , Cat. No. 565388 . Two-color flow cytometric contour plots showing the correlated expression of Violet Live Caspase Probe fluorescence versus BD Horizon Fixable Viability Stain 780 fluorescence were derived from gated events with the forward and side light-scatter characteristics of viable cells. Live cells are double negative for both fluorescent probes, whereas apoptotic cells are positive for Violet Live Cell Caspase Probe and negative for FVS780, and dead cells are positive for FVS780. Note that dead cells bind intermediate amounts of Violet Live Cell Caspase Probe. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Violet Live Cell Caspase Probe has been tested on mouse data not shown . Show More Two-color flow cytometric analysis of Viability and Caspase Activity in Camptothecin-treated Jurkat Cells. Cells from the human Jurkat Acute T cell leukemia, ATCC TIB-152 cell line were treated with 0.025 % DMSO Left Panel or 5 muM Camptothecin Right Panel for 16 hours. The cultured cells were then stained with BD Pharmingen Violet Live Cell Caspase Probe Cat. No. 565521 according to the recommended assay procedure. After recovery in media, cells were resuspended in DPBS and stained with BD Horizon Fixable Viability Stain 780 FVS780 , Cat. No. 565388 . Two-color flow cytometric contour plots showing the correlated expression of Violet Live Caspase Probe fluorescence versus BD Horizon Fixable Viability Stain 780 fluorescence were derived from gated events with the forward and side light-scatter characteristics of viable cells. Live cells are double negative for both fluorescent probes, whereas apoptotic cells are positive for Violet Live Cell Caspase Probe and negative for FVS780, and dead cells are positive for FVS780. Note that dead cells bind intermediate amounts of Violet Live Cell Caspase Probe. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Violet Live Cell Caspase Probe has been tested on mouse data not shown . Two-color flow cytometric analysis of Viability and Caspase Activity in Camptothecin-treated Jurkat Cells. Cells from the human Jurkat Acute T cell leukemia, ATCC TIB-152 cell line were treated with 0.025 % DMSO Left Panel or 5 muM Camptothecin Right Panel for 16 hours. The cultured cells were then stained with BD Pharmingen Violet Live Cell Caspase Probe Cat. No. 565521 according to the recommended assay procedure. After recovery in media, cells were resuspended in DPBS and stained with BD Horizon Fixable Viability Stain 780 FVS780 , Cat. No. 565388 . Two-color flow cytometric contour plots showing the correlated expression of Violet Live Caspase Probe fluorescence versus BD Horizon Fixable Viability Stain 780 fluorescence were derived from gated events with the forward and side light-scatter characteristics of viable cells. Live cells are double negative for both fluorescent probes, whereas apoptotic cells are positive for Violet Live Cell Caspase Probe and negative for FVS780, and dead cells are positive for FVS780. Note that dead cells bind intermediate amounts of Violet Live Cell Caspase Probe. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Violet Live Cell Caspase Probe has been tested on mouse data not shown .