BD PhosflowTM Alexa Fluor 488 Mouse anti-Akt1

Description

Analysis of Akt1 in human epithelioid carcinoma. HeLa S3 cells ATCC CCL 2.2 were either transfected with Akt1 RNAi open histogram or untreated shaded histogram . The cells were fixed BD Cytofix buffer, Cat. No. 554655 for 10 minutes at 37 C, then permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for 30 minutes, and then stained with Alexa Fluor 488 Mouse anti- Akt1 Cat. No. 560048 . Down-regulation of Akt1 expression is evident in the RNAi-transfected cells. Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Analysis of Akt1 in human epithelioid carcinoma. HeLa S3 cells ATCC CCL 2.2 were either transfected with Akt1 RNAi open histogram or untreated shaded histogram . The cells were fixed BD Cytofix buffer, Cat. No. 554655 for 10 minutes at 37 C, then permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for 30 minutes, and then stained with Alexa Fluor 488 Mouse anti- Akt1 Cat. No. 560048 . Down-regulation of Akt1 expression is evident in the RNAi-transfected cells. Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Show More Analysis of Akt1 in human epithelioid carcinoma. HeLa S3 cells ATCC CCL 2.2 were either transfected with Akt1 RNAi open histogram or untreated shaded histogram . The cells were fixed BD Cytofix buffer, Cat. No. 554655 for 10 minutes at 37 C, then permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for 30 minutes, and then stained with Alexa Fluor 488 Mouse anti- Akt1 Cat. No. 560048 . Down-regulation of Akt1 expression is evident in the RNAi-transfected cells. Flow cytometry was performed on a BD FACSCalibur flow cytometry system.