BD PhosflowTM Alexa Fluor 488 Mouse anti-Ezrin (pY353)

Description

Analysis of Ezrin pY353 in human chronic myelogenous leukemia. Serum-starved K-562 cells ATCC CCL-243 were either treated with 1 mM Pervanadate Sigma Cat. No. S6508 at 37 C for 20 minutes shaded histogram or untreated open histogram . The cells were fixed BD Cytofix buffer, Cat. No. 554655 for 10 minutes at 37 C, then permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for at least 30 minutes, and then stained with Alexa Fluor 488 Mouse anti-Ezrin pY353, Cat. No. 558627 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Analysis of Ezrin pY353 in human chronic myelogenous leukemia. Serum-starved K-562 cells ATCC CCL-243 were either treated with 1 mM Pervanadate Sigma Cat. No. S6508 at 37 C for 20 minutes shaded histogram or untreated open histogram . The cells were fixed BD Cytofix buffer, Cat. No. 554655 for 10 minutes at 37 C, then permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for at least 30 minutes, and then stained with Alexa Fluor 488 Mouse anti-Ezrin pY353, Cat. No. 558627 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Show More Analysis of Ezrin pY353 in human chronic myelogenous leukemia. Serum-starved K-562 cells ATCC CCL-243 were either treated with 1 mM Pervanadate Sigma Cat. No. S6508 at 37 C for 20 minutes shaded histogram or untreated open histogram . The cells were fixed BD Cytofix buffer, Cat. No. 554655 for 10 minutes at 37 C, then permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for at least 30 minutes, and then stained with Alexa Fluor 488 Mouse anti-Ezrin pY353, Cat. No. 558627 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system.