BD PhosflowTM Alexa Fluor 488 Mouse Anti-LCK (pY505)

Description

Flow cytometric analysis of Phospho-Lck pY505 Alexa 488. Jurkat T cells ATCC TIB 152 were either left untreated unshaded or treated shaded with H2O2 5 mM for 15 minutes at 37C . Cells were fixed in BD Cytofix buffer Cat. No. 554655 and permeabilized using BD Phosflow Perm Buffer III Cat. No. 558050 and then stained with Alexa Fluor 488 Mouse Anti-LCK pY505 1 hour at room temperature; Cat. No. 557879 . The cells were analyzed on a BD FACSCalibur flow cytometry system. Flow cytometric analysis of Phospho-Lck pY505 Alexa 488. Jurkat T cells ATCC TIB 152 were either left untreated unshaded or treated shaded with H2O2 5 mM for 15 minutes at 37C . Cells were fixed in BD Cytofix buffer Cat. No. 554655 and permeabilized using BD Phosflow Perm Buffer III Cat. No. 558050 and then stained with Alexa Fluor 488 Mouse Anti-LCK pY505 1 hour at room temperature; Cat. No. 557879 . The cells were analyzed on a BD FACSCalibur flow cytometry system. Show More Flow cytometric analysis of Phospho-Lck pY505 Alexa 488. Jurkat T cells ATCC TIB 152 were either left untreated unshaded or treated shaded with H2O2 5 mM for 15 minutes at 37C . Cells were fixed in BD Cytofix buffer Cat. No. 554655 and permeabilized using BD Phosflow Perm Buffer III Cat. No. 558050 and then stained with Alexa Fluor 488 Mouse Anti-LCK pY505 1 hour at room temperature; Cat. No. 557879 . The cells were analyzed on a BD FACSCalibur flow cytometry system.