BD PhosflowTM Alexa Fluor 488 Mouse anti-PDGFRbeta (CD140b) (pY771)

Description

Analysis of PDGFRbeta CD140b pY771 in mouse embryonic fibroblasts. Serum-starved NIH/3T3 cells were either stimulated with PDGF-BB shaded histogram or unstimulated open histogram . The cells were fixed BD Phosflow Fix Buffer I, Cat. No. 557870 for 10 minutes at 37 C, then permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for at least 30 minutes, and then stained with Alexa Fluor 488 anti-PDGFRbeta CD140b, pY771, Cat. No. 558424 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Analysis of PDGFRbeta CD140b pY771 in mouse embryonic fibroblasts. Serum-starved NIH/3T3 cells were either stimulated with PDGF-BB shaded histogram or unstimulated open histogram . The cells were fixed BD Phosflow Fix Buffer I, Cat. No. 557870 for 10 minutes at 37 C, then permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for at least 30 minutes, and then stained with Alexa Fluor 488 anti-PDGFRbeta CD140b, pY771, Cat. No. 558424 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Show More Analysis of PDGFRbeta CD140b pY771 in mouse embryonic fibroblasts. Serum-starved NIH/3T3 cells were either stimulated with PDGF-BB shaded histogram or unstimulated open histogram . The cells were fixed BD Phosflow Fix Buffer I, Cat. No. 557870 for 10 minutes at 37 C, then permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for at least 30 minutes, and then stained with Alexa Fluor 488 anti-PDGFRbeta CD140b, pY771, Cat. No. 558424 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system.