BD PhosflowTM Alexa Fluor 488 Mouse anti-PLC-gamma2

Description

Analysis of PLC-gamma2 in human peripheral blood lymphocytes. Human whole blood was lysed and fixed with 1X BD Phosflow Lyse/Fix Buffer Cat. No. 558049 for 10-15 minutes at 37oC, then permeabilized BD Phosflow Perm Buffer II, Cat. No. 558052 on ice for 30 minutes, and then stained with Alexa Fluor 647 Mouse Anti-Human CD3 Cat. No. 557706 , PerCP-Cy5.5 anti-human CD20 Cat. No. 558021 , and either Alexa Fluor 488 Mouse anti-PLC-gamma2 Cat. No. 560135; solid-line histograms or Alexa Fluor 488 Mouse IgG1, kappa Isotype control Cat. No. 557782, dashed line histograms . The figures show lymphocyte subpopulations that were selected by their scatter profile and surface antigen expression. PLC-gamma2 expression on CD20-positive CD3-negative B lymphocytes left panel and CD20-negative CD3-negative NK cells right panel are displayed. There was little detectable expression of PLC-gamma2 in the CD20-negative CD3-positive T lymphocytes not shown . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Analysis of PLC-gamma2 in human peripheral blood lymphocytes. Human whole blood was lysed and fixed with 1X BD Phosflow Lyse/Fix Buffer Cat. No. 558049 for 10-15 minutes at 37oC, then permeabilized BD Phosflow Perm Buffer II, Cat. No. 558052 on ice for 30 minutes, and then stained with Alexa Fluor 647 Mouse Anti-Human CD3 Cat. No. 557706 , PerCP-Cy5.5 anti-human CD20 Cat. No. 558021 , and either Alexa Fluor 488 Mouse anti-PLC-gamma2 Cat. No. 560135; solid-line histograms or Alexa Fluor 488 Mouse IgG1, kappa Isotype control Cat. No. 557782, dashed line histograms . The figures show lymphocyte subpopulations that were selected by their scatter profile and surface antigen expression. PLC-gamma2 expression on CD20-positive CD3-negative B lymphocytes left panel and CD20-negative CD3-negative NK cells right panel are displayed. There was little detectable expression of PLC-gamma2 in the CD20-negative CD3-positive T lymphocytes not shown . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Show More Analysis of PLC-gamma2 in human peripheral blood lymphocytes. Human whole blood was lysed and fixed with 1X BD Phosflow Lyse/Fix Buffer Cat. No. 558049 for 10-15 minutes at 37oC, then permeabilized BD Phosflow Perm Buffer II, Cat. No. 558052 on ice for 30 minutes, and then stained with Alexa Fluor 647 Mouse Anti-Human CD3 Cat. No. 557706 , PerCP-Cy5.5 anti-human CD20 Cat. No. 558021 , and either Alexa Fluor 488 Mouse anti-PLC-gamma2 Cat. No. 560135; solid-line histograms or Alexa Fluor 488 Mouse IgG1, kappa Isotype control Cat. No. 557782, dashed line histograms . The figures show lymphocyte subpopulations that were selected by their scatter profile and surface antigen expression. PLC-gamma2 expression on CD20-positive CD3-negative B lymphocytes left panel and CD20-negative CD3-negative NK cells right panel are displayed. There was little detectable expression of PLC-gamma2 in the CD20-negative CD3-positive T lymphocytes not shown . Flow cytometry was performed on a BD FACSCalibur flow cytometry system.