BD PhosflowTM Alexa Fluor 488 Mouse anti-PLK1 (pT210)

Description

Analysis of PLK1 pT210 in human epithelioid carcinoma. Hela S3 cells ATCC CCL 2.2 were either stimulated with Nocodazole at 37 C for 12-16 hours shaded histogram or unstimulated open histogram . The cells were fixed BD Cytofix buffer, Cat. No. 554655 for 10 minutes at 37 C, then permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for at least 30 minutes, and then stained with Alexa Fluor 488 Mouse anti-PLK1 pT210 . Simultaneous staining with 7-AAD Cat. No. 559925 demonstrates that the cells with upregulated PLK1 phosphorylation are in the G2/M phase of the cell cycle data not shown . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Analysis of PLK1 pT210 in human epithelioid carcinoma. Hela S3 cells ATCC CCL 2.2 were either stimulated with Nocodazole at 37 C for 12-16 hours shaded histogram or unstimulated open histogram . The cells were fixed BD Cytofix buffer, Cat. No. 554655 for 10 minutes at 37 C, then permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for at least 30 minutes, and then stained with Alexa Fluor 488 Mouse anti-PLK1 pT210 . Simultaneous staining with 7-AAD Cat. No. 559925 demonstrates that the cells with upregulated PLK1 phosphorylation are in the G2/M phase of the cell cycle data not shown . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Show More Analysis of PLK1 pT210 in human epithelioid carcinoma. Hela S3 cells ATCC CCL 2.2 were either stimulated with Nocodazole at 37 C for 12-16 hours shaded histogram or unstimulated open histogram . The cells were fixed BD Cytofix buffer, Cat. No. 554655 for 10 minutes at 37 C, then permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for at least 30 minutes, and then stained with Alexa Fluor 488 Mouse anti-PLK1 pT210 . Simultaneous staining with 7-AAD Cat. No. 559925 demonstrates that the cells with upregulated PLK1 phosphorylation are in the G2/M phase of the cell cycle data not shown . Flow cytometry was performed on a BD FACSCalibur flow cytometry system.