BD PhosflowTM Alexa Fluor 488 Mouse anti-Syk (pY348)

Description

Flow cytometric analysis for Syk pY348 . Jurkat cells Human T-cell leukemia; ATCC TIB-152 were serum starved overnight, then either treated with 5 mM H2O2 at 37C for 15 minutes shaded histogram or were untreated open histogram . The cells were harvested and fixed with pre-warmed BD Cytofix Fixation Buffer Cat. No. 554655 for 10 minutes at 37C, permeabilized with BD Phosflow Perm buffer II Cat. No. 558052 on ice for 30 minutes, and then stained with Alexa Fluor 488 Mouse anti-Syk pY348 . The histograms were derived from the light scattering characteristics of the viable cells. The data demonstrates the upregulated phosphorylation of the treated cells versus untreated cells. Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Flow cytometric analysis for Syk pY348 . Jurkat cells Human T-cell leukemia; ATCC TIB-152 were serum starved overnight, then either treated with 5 mM H2O2 at 37C for 15 minutes shaded histogram or were untreated open histogram . The cells were harvested and fixed with pre-warmed BD Cytofix Fixation Buffer Cat. No. 554655 for 10 minutes at 37C, permeabilized with BD Phosflow Perm buffer II Cat. No. 558052 on ice for 30 minutes, and then stained with Alexa Fluor 488 Mouse anti-Syk pY348 . The histograms were derived from the light scattering characteristics of the viable cells. The data demonstrates the upregulated phosphorylation of the treated cells versus untreated cells. Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Show More Flow cytometric analysis for Syk pY348 . Jurkat cells Human T-cell leukemia; ATCC TIB-152 were serum starved overnight, then either treated with 5 mM H2O2 at 37C for 15 minutes shaded histogram or were untreated open histogram . The cells were harvested and fixed with pre-warmed BD Cytofix Fixation Buffer Cat. No. 554655 for 10 minutes at 37C, permeabilized with BD Phosflow Perm buffer II Cat. No. 558052 on ice for 30 minutes, and then stained with Alexa Fluor 488 Mouse anti-Syk pY348 . The histograms were derived from the light scattering characteristics of the viable cells. The data demonstrates the upregulated phosphorylation of the treated cells versus untreated cells. Flow cytometry was performed on a BD FACSCalibur flow cytometry system.