BD PhosflowTM Alexa Fluor 488 Mouse anti-TBK1

Description

Analysis of TBK1 in transfected human epithelial cells. The 293 fetal kidney cell line left panel was either co-transfected with TBK1 and IRF-7 expression vectors dashed histogram or un-transfected solid line . After 24 hours, the cells were fixed BD Cytofix buffer, Cat. No. 554655 for 10 minutes at 37 C, then permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for at least 30 minutes, and then stained with Alexa Fluor 488 Mouse anti-TBK1 Cat. No. 558697 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. The specificity of mAb 637Ig11.2 was confirmed by western blot right panel using unconjugated antibody on lysates from untransfected left panel and TBK1/IRF-7 co-transfected right panel 293 cells. The TBK1-Myc tagged fusion protein is identified as a band of 84 kDa in the lysate of co-transfected cells. Analysis of TBK1 in transfected human epithelial cells. The 293 fetal kidney cell line left panel was either co-transfected with TBK1 and IRF-7 expression vectors dashed histogram or un-transfected solid line . After 24 hours, the cells were fixed BD Cytofix buffer, Cat. No. 554655 for 10 minutes at 37 C, then permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for at least 30 minutes, and then stained with Alexa Fluor 488 Mouse anti-TBK1 Cat. No. 558697 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. The specificity of mAb 637Ig11.2 was confirmed by western blot right panel using unconjugated antibody on lysates from untransfected left panel and TBK1/IRF-7 co-transfected right panel 293 cells. The TBK1-Myc tagged fusion protein is identified as a band of 84 kDa in the lysate of co-transfected cells. Analysis of TBK1 in transfected human epithelial cells. The 293 fetal kidney cell line left panel was either co-transfected with TBK1 and IRF-7 expression vectors dashed histogram or un-transfected solid line . After 24 hours, the cells were fixed BD Cytofix buffer, Cat. No. 554655 for 10 minutes at 37 C, then permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for at least 30 minutes, and then stained with Alexa Fluor 488 Mouse anti-TBK1 Cat. No. 558697 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. The specificity of mAb 637Ig11.2 was confirmed by western blot right panel using unconjugated antibody on lysates from untransfected left panel and TBK1/IRF-7 co-transfected right panel 293 cells. The TBK1-Myc tagged fusion protein is identified as a band of 84 kDa in the lysate of co-transfected cells. Show More Analysis of TBK1 in transfected human epithelial cells. The 293 fetal kidney cell line left panel was either co-transfected with TBK1 and IRF-7 expression vectors dashed histogram or un-transfected solid line . After 24 hours, the cells were fixed BD Cytofix buffer, Cat. No. 554655 for 10 minutes at 37 C, then permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for at least 30 minutes, and then stained with Alexa Fluor 488 Mouse anti-TBK1 Cat. No. 558697 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. The specificity of mAb 637Ig11.2 was confirmed by western blot right panel using unconjugated antibody on lysates from untransfected left panel and TBK1/IRF-7 co-transfected right panel 293 cells. The TBK1-Myc tagged fusion protein is identified as a band of 84 kDa in the lysate of co-transfected cells. Show More Analysis of TBK1 in transfected human epithelial cells. The 293 fetal kidney cell line left panel was either co-transfected with TBK1 and IRF-7 expression vectors dashed histogram or un-transfected solid line . After 24 hours, the cells were fixed BD Cytofix buffer, Cat. No. 554655 for 10 minutes at 37 C, then permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for at least 30 minutes, and then stained with Alexa Fluor 488 Mouse anti-TBK1 Cat. No. 558697 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. The specificity of mAb 637Ig11.2 was confirmed by western blot right panel using unconjugated antibody on lysates from untransfected left panel and TBK1/IRF-7 co-transfected right panel 293 cells. The TBK1-Myc tagged fusion protein is identified as a band of 84 kDa in the lysate of co-transfected cells. Analysis of TBK1 in transfected human epithelial cells. The 293 fetal kidney cell line left panel was either co-transfected with TBK1 and IRF-7 expression vectors dashed histogram or un-transfected solid line . After 24 hours, the cells were fixed BD Cytofix buffer, Cat. No. 554655 for 10 minutes at 37 C, then permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for at least 30 minutes, and then stained with Alexa Fluor 488 Mouse anti-TBK1 Cat. No. 558697 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. The specificity of mAb 637Ig11.2 was confirmed by western blot right panel using unconjugated antibody on lysates from untransfected left panel and TBK1/IRF-7 co-transfected right panel 293 cells. The TBK1-Myc tagged fusion protein is identified as a band of 84 kDa in the lysate of co-transfected cells.