BD PhosflowTM Alexa Fluor 488 Mouse anti-TIE2 (pY1102)

Description

Analysis of TIE2 pY1102 in human vascular endothelium. EA-hy 926 cells Edgell, McDonald, Graham, 1983 were serum starved overnight, detached using 1X trypsin, washed, resuspended in serum-free DMEM and rested for 20 minutes at 37 C, and then either left unstimulated open histogram or stimulated with 1 mM Pervanadate Sigma Cat. No. S6508, shaded histogram for 20 minutes at 37 C left figure . The cells were fixed BD Cytofix buffer, Cat. No. 554655 for 10 minutes at 37 C, then permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for at least 30 minutes, and then stained with Alexa Fluor 488 Mouse anti-TIE2 pY1102, Cat. No. 560053 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. The specificity of mAb K93-754 was confirmed by western blot using unconjugated antibody, at 0.25, 0.125 and 0.063 mug/ml lanes 1, 2, and 3, respectively on lysates from control left panel and Pervanadate-treated right panel EA-hy 926 cells right figure . TIE2 pY1102 is identified as a band of 160 kDa in the treated cells. Analysis of TIE2 pY1102 in human vascular endothelium. EA-hy 926 cells Edgell, McDonald, Graham, 1983 were serum starved overnight, detached using 1X trypsin, washed, resuspended in serum-free DMEM and rested for 20 minutes at 37 C, and then either left unstimulated open histogram or stimulated with 1 mM Pervanadate Sigma Cat. No. S6508, shaded histogram for 20 minutes at 37 C left figure . The cells were fixed BD Cytofix buffer, Cat. No. 554655 for 10 minutes at 37 C, then permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for at least 30 minutes, and then stained with Alexa Fluor 488 Mouse anti-TIE2 pY1102, Cat. No. 560053 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. The specificity of mAb K93-754 was confirmed by western blot using unconjugated antibody, at 0.25, 0.125 and 0.063 mug/ml lanes 1, 2, and 3, respectively on lysates from control left panel and Pervanadate-treated right panel EA-hy 926 cells right figure . TIE2 pY1102 is identified as a band of 160 kDa in the treated cells. Analysis of TIE2 pY1102 in human vascular endothelium. EA-hy 926 cells Edgell, McDonald, Graham, 1983 were serum starved overnight, detached using 1X trypsin, washed, resuspended in serum-free DMEM and rested for 20 minutes at 37 C, and then either left unstimulated open histogram or stimulated with 1 mM Pervanadate Sigma Cat. No. S6508, shaded histogram for 20 minutes at 37 C left figure . The cells were fixed BD Cytofix buffer, Cat. No. 554655 for 10 minutes at 37 C, then permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for at least 30 minutes, and then stained with Alexa Fluor 488 Mouse anti-TIE2 pY1102, Cat. No. 560053 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. The specificity of mAb K93-754 was confirmed by western blot using unconjugated antibody, at 0.25, 0.125 and 0.063 mug/ml lanes 1, 2, and 3, respectively on lysates from control left panel and Pervanadate-treated right panel EA-hy 926 cells right figure . TIE2 pY1102 is identified as a band of 160 kDa in the treated cells. Show More Analysis of TIE2 pY1102 in human vascular endothelium. EA-hy 926 cells Edgell, McDonald, Graham, 1983 were serum starved overnight, detached using 1X trypsin, washed, resuspended in serum-free DMEM and rested for 20 minutes at 37 C, and then either left unstimulated open histogram or stimulated with 1 mM Pervanadate Sigma Cat. No. S6508, shaded histogram for 20 minutes at 37 C left figure . The cells were fixed BD Cytofix buffer, Cat. No. 554655 for 10 minutes at 37 C, then permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for at least 30 minutes, and then stained with Alexa Fluor 488 Mouse anti-TIE2 pY1102, Cat. No. 560053 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. The specificity of mAb K93-754 was confirmed by western blot using unconjugated antibody, at 0.25, 0.125 and 0.063 mug/ml lanes 1, 2, and 3, respectively on lysates from control left panel and Pervanadate-treated right panel EA-hy 926 cells right figure . TIE2 pY1102 is identified as a band of 160 kDa in the treated cells. Show More Analysis of TIE2 pY1102 in human vascular endothelium. EA-hy 926 cells Edgell, McDonald, Graham, 1983 were serum starved overnight, detached using 1X trypsin, washed, resuspended in serum-free DMEM and rested for 20 minutes at 37 C, and then either left unstimulated open histogram or stimulated with 1 mM Pervanadate Sigma Cat. No. S6508, shaded histogram for 20 minutes at 37 C left figure . The cells were fixed BD Cytofix buffer, Cat. No. 554655 for 10 minutes at 37 C, then permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for at least 30 minutes, and then stained with Alexa Fluor 488 Mouse anti-TIE2 pY1102, Cat. No. 560053 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. The specificity of mAb K93-754 was confirmed by western blot using unconjugated antibody, at 0.25, 0.125 and 0.063 mug/ml lanes 1, 2, and 3, respectively on lysates from control left panel and Pervanadate-treated right panel EA-hy 926 cells right figure . TIE2 pY1102 is identified as a band of 160 kDa in the treated cells. Analysis of TIE2 pY1102 in human vascular endothelium. EA-hy 926 cells Edgell, McDonald, Graham, 1983 were serum starved overnight, detached using 1X trypsin, washed, resuspended in serum-free DMEM and rested for 20 minutes at 37 C, and then either left unstimulated open histogram or stimulated with 1 mM Pervanadate Sigma Cat. No. S6508, shaded histogram for 20 minutes at 37 C left figure . The cells were fixed BD Cytofix buffer, Cat. No. 554655 for 10 minutes at 37 C, then permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for at least 30 minutes, and then stained with Alexa Fluor 488 Mouse anti-TIE2 pY1102, Cat. No. 560053 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. The specificity of mAb K93-754 was confirmed by western blot using unconjugated antibody, at 0.25, 0.125 and 0.063 mug/ml lanes 1, 2, and 3, respectively on lysates from control left panel and Pervanadate-treated right panel EA-hy 926 cells right figure . TIE2 pY1102 is identified as a band of 160 kDa in the treated cells.