BD PhosflowTM Alexa Fluor 647 Mouse anti-BLNK (pY84)

Description

Analysis of BLNK pY84 in human Burkitts lymphoma and peripheral blood lymphocytes. Ramos cells left panel and human peripheral blood mononuclear cells PBMC, right panel were either stimulated solid-line histograms by cross-linking of surface IgM with purified Goat anti-Human IgM SouthernBiotech at 37C for 2 minutes or unstimulated dashed-line histograms . The cells were fixed with pre-warmed BD Cytofix buffer Cat. No. 554655 for 10 minutes at 37oC, then permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 at room temperature for 30 minutes, and then stained with Alexa Fluor 647 anti-BLNK pY84 both panels , APC anti-human CD3 mAb UCHT1 Cat. No. 555335, bottom panel , and PerCP-Cy5.5 anti-human CD20 mAb H1 FB1 Cat. No. 558021, bottom panel . For data analysis of PBMC, CD3-negative CD20-positive lymphocytes were selected by their staining and scatter profiles. Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Analysis of BLNK pY84 in human Burkitts lymphoma and peripheral blood lymphocytes. Ramos cells left panel and human peripheral blood mononuclear cells PBMC, right panel were either stimulated solid-line histograms by cross-linking of surface IgM with purified Goat anti-Human IgM SouthernBiotech at 37C for 2 minutes or unstimulated dashed-line histograms . The cells were fixed with pre-warmed BD Cytofix buffer Cat. No. 554655 for 10 minutes at 37oC, then permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 at room temperature for 30 minutes, and then stained with Alexa Fluor 647 anti-BLNK pY84 both panels , APC anti-human CD3 mAb UCHT1 Cat. No. 555335, bottom panel , and PerCP-Cy5.5 anti-human CD20 mAb H1 FB1 Cat. No. 558021, bottom panel . For data analysis of PBMC, CD3-negative CD20-positive lymphocytes were selected by their staining and scatter profiles. Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Analysis of BLNK pY84 in human Burkitts lymphoma and peripheral blood lymphocytes. Ramos cells left panel and human peripheral blood mononuclear cells PBMC, right panel were either stimulated solid-line histograms by cross-linking of surface IgM with purified Goat anti-Human IgM SouthernBiotech at 37C for 2 minutes or unstimulated dashed-line histograms . The cells were fixed with pre-warmed BD Cytofix buffer Cat. No. 554655 for 10 minutes at 37oC, then permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 at room temperature for 30 minutes, and then stained with Alexa Fluor 647 anti-BLNK pY84 both panels , APC anti-human CD3 mAb UCHT1 Cat. No. 555335, bottom panel , and PerCP-Cy5.5 anti-human CD20 mAb H1 FB1 Cat. No. 558021, bottom panel . For data analysis of PBMC, CD3-negative CD20-positive lymphocytes were selected by their staining and scatter profiles. Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Show More Analysis of BLNK pY84 in human Burkitts lymphoma and peripheral blood lymphocytes. Ramos cells left panel and human peripheral blood mononuclear cells PBMC, right panel were either stimulated solid-line histograms by cross-linking of surface IgM with purified Goat anti-Human IgM SouthernBiotech at 37C for 2 minutes or unstimulated dashed-line histograms . The cells were fixed with pre-warmed BD Cytofix buffer Cat. No. 554655 for 10 minutes at 37oC, then permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 at room temperature for 30 minutes, and then stained with Alexa Fluor 647 anti-BLNK pY84 both panels , APC anti-human CD3 mAb UCHT1 Cat. No. 555335, bottom panel , and PerCP-Cy5.5 anti-human CD20 mAb H1 FB1 Cat. No. 558021, bottom panel . For data analysis of PBMC, CD3-negative CD20-positive lymphocytes were selected by their staining and scatter profiles. Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Show More Analysis of BLNK pY84 in human Burkitts lymphoma and peripheral blood lymphocytes. Ramos cells left panel and human peripheral blood mononuclear cells PBMC, right panel were either stimulated solid-line histograms by cross-linking of surface IgM with purified Goat anti-Human IgM SouthernBiotech at 37C for 2 minutes or unstimulated dashed-line histograms . The cells were fixed with pre-warmed BD Cytofix buffer Cat. No. 554655 for 10 minutes at 37oC, then permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 at room temperature for 30 minutes, and then stained with Alexa Fluor 647 anti-BLNK pY84 both panels , APC anti-human CD3 mAb UCHT1 Cat. No. 555335, bottom panel , and PerCP-Cy5.5 anti-human CD20 mAb H1 FB1 Cat. No. 558021, bottom panel . For data analysis of PBMC, CD3-negative CD20-positive lymphocytes were selected by their staining and scatter profiles. Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Analysis of BLNK pY84 in human Burkitts lymphoma and peripheral blood lymphocytes. Ramos cells left panel and human peripheral blood mononuclear cells PBMC, right panel were either stimulated solid-line histograms by cross-linking of surface IgM with purified Goat anti-Human IgM SouthernBiotech at 37C for 2 minutes or unstimulated dashed-line histograms . The cells were fixed with pre-warmed BD Cytofix buffer Cat. No. 554655 for 10 minutes at 37oC, then permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 at room temperature for 30 minutes, and then stained with Alexa Fluor 647 anti-BLNK pY84 both panels , APC anti-human CD3 mAb UCHT1 Cat. No. 555335, bottom panel , and PerCP-Cy5.5 anti-human CD20 mAb H1 FB1 Cat. No. 558021, bottom panel . For data analysis of PBMC, CD3-negative CD20-positive lymphocytes were selected by their staining and scatter profiles. Flow cytometry was performed on a BD FACSCalibur flow cytometry system.