BD PhosflowTM Alexa Fluor 647 Mouse anti-Btk

Description

Analysis of Btk in human peripheral blood lymphocytes. Human whole blood was lysed and fixed with 1X BD Phosflow Lyse/Fix Buffer Cat. No. 558049 for 10-15 minutes at 37oC, then permeabilized BD Phosflow Perm Buffer II, Cat. No. 558052 on ice for 30 minutes, and then stained with either Alexa Fluor 647 Mouse anti-Btk Cat. No. 558528, solid-line histogram or Alexa Fluor 647 Mouse IgG2a isotype control mAb MOPC-173 Cat. No. 558053, dashed-line histogram . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. The figure displays lymphocytes that were selected by their scatter profile, and the cells showing higher Btk expression are B lymphocytes. Analysis of Btk in human peripheral blood lymphocytes. Human whole blood was lysed and fixed with 1X BD Phosflow Lyse/Fix Buffer Cat. No. 558049 for 10-15 minutes at 37oC, then permeabilized BD Phosflow Perm Buffer II, Cat. No. 558052 on ice for 30 minutes, and then stained with either Alexa Fluor 647 Mouse anti-Btk Cat. No. 558528, solid-line histogram or Alexa Fluor 647 Mouse IgG2a isotype control mAb MOPC-173 Cat. No. 558053, dashed-line histogram . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. The figure displays lymphocytes that were selected by their scatter profile, and the cells showing higher Btk expression are B lymphocytes. Show More Analysis of Btk in human peripheral blood lymphocytes. Human whole blood was lysed and fixed with 1X BD Phosflow Lyse/Fix Buffer Cat. No. 558049 for 10-15 minutes at 37oC, then permeabilized BD Phosflow Perm Buffer II, Cat. No. 558052 on ice for 30 minutes, and then stained with either Alexa Fluor 647 Mouse anti-Btk Cat. No. 558528, solid-line histogram or Alexa Fluor 647 Mouse IgG2a isotype control mAb MOPC-173 Cat. No. 558053, dashed-line histogram . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. The figure displays lymphocytes that were selected by their scatter profile, and the cells showing higher Btk expression are B lymphocytes.