BD PhosflowTM Alexa Fluor 647 Mouse Anti-Human PLCgamma1 (pY783)

Description

Analysis of PLC gamma 1 pY783 in activated human T leukemia cells. Jurkat cells ATCC TIB152 were either stimulated with 5 mM hydrogen peroxide for 15 minutes shaded histogram or unstimulated open histogram . The cells were fixed BD Cytofix Fixation Buffer, Cat. No. 554655 for 10 minutes, then permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for at least 30 minutes, and then stained with Alexa Fluor 647 anti-PLC gamma 1 pY783 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Analysis of PLC gamma 1 pY783 in activated human T leukemia cells. Jurkat cells ATCC TIB152 were either stimulated with 5 mM hydrogen peroxide for 15 minutes shaded histogram or unstimulated open histogram . The cells were fixed BD Cytofix Fixation Buffer, Cat. No. 554655 for 10 minutes, then permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for at least 30 minutes, and then stained with Alexa Fluor 647 anti-PLC gamma 1 pY783 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Show More Analysis of PLC gamma 1 pY783 in activated human T leukemia cells. Jurkat cells ATCC TIB152 were either stimulated with 5 mM hydrogen peroxide for 15 minutes shaded histogram or unstimulated open histogram . The cells were fixed BD Cytofix Fixation Buffer, Cat. No. 554655 for 10 minutes, then permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for at least 30 minutes, and then stained with Alexa Fluor 647 anti-PLC gamma 1 pY783 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system.