BD PhosflowTM Alexa Fluor 647 Mouse anti-IGF-1 Receptor (CD221) (pY1131)

Description

Analysis of IGF-1 Receptor pY1131 in transformed human epithelial cells. Serum-starved 293 fetal kidney cells were either stimulated at 37C for 2 minutes with 100 nM IGF-I shaded histogram or unstimulated open histogram . The cells were fixed with pre-warmed BD Cytofix buffer Cat. No. 554655 for 10 minutes at 37 C, then permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for at least 30 minutes, and then stained with Alexa Fluor 647 Mouse anti-IGF-1 Receptor CD221 pY1131, Cat. No. 558588 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Analysis of IGF-1 Receptor pY1131 in transformed human epithelial cells. Serum-starved 293 fetal kidney cells were either stimulated at 37C for 2 minutes with 100 nM IGF-I shaded histogram or unstimulated open histogram . The cells were fixed with pre-warmed BD Cytofix buffer Cat. No. 554655 for 10 minutes at 37 C, then permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for at least 30 minutes, and then stained with Alexa Fluor 647 Mouse anti-IGF-1 Receptor CD221 pY1131, Cat. No. 558588 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Show More Analysis of IGF-1 Receptor pY1131 in transformed human epithelial cells. Serum-starved 293 fetal kidney cells were either stimulated at 37C for 2 minutes with 100 nM IGF-I shaded histogram or unstimulated open histogram . The cells were fixed with pre-warmed BD Cytofix buffer Cat. No. 554655 for 10 minutes at 37 C, then permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for at least 30 minutes, and then stained with Alexa Fluor 647 Mouse anti-IGF-1 Receptor CD221 pY1131, Cat. No. 558588 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system.