BD PhosflowTM Alexa Fluor 647 Mouse anti-p53 (acK382)

Description

Analysis of p53 acK382 in lymphocytes. Human peripheral blood mononuclear cells PBMC were either treated with 0.4 muM Trichostatin A Sigma, Cat. No. T8552 plus 0.4 muM Adriamycin Doxorubicin hydrochloride, Sigma, Cat. No. D1515 for 24 hours shaded histogram or untreated open histogram . The cells were fixed BD Cytofix buffer, Cat. No. 554655 for 10 minutes, then permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for at least 30 minutes, and then stained with Alexa Fluor 647 Mouse anti-p53 acK382 . Lymphocytes were selected by scatter profile. Flow cytometry was performed on a BD FACSArray bioanalyzer system. The specificity of mAb L82-51 was confirmed by western blot analysis using unconjugated antibody on lysates from control left blot and Trichostatin A-plus-Adriamycin-treated right blot PBMC. p53 acK382 is identified as a band of 53 kDa in the treated cells. Analysis of p53 acK382 in lymphocytes. Human peripheral blood mononuclear cells PBMC were either treated with 0.4 muM Trichostatin A Sigma, Cat. No. T8552 plus 0.4 muM Adriamycin Doxorubicin hydrochloride, Sigma, Cat. No. D1515 for 24 hours shaded histogram or untreated open histogram . The cells were fixed BD Cytofix buffer, Cat. No. 554655 for 10 minutes, then permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for at least 30 minutes, and then stained with Alexa Fluor 647 Mouse anti-p53 acK382 . Lymphocytes were selected by scatter profile. Flow cytometry was performed on a BD FACSArray bioanalyzer system. The specificity of mAb L82-51 was confirmed by western blot analysis using unconjugated antibody on lysates from control left blot and Trichostatin A-plus-Adriamycin-treated right blot PBMC. p53 acK382 is identified as a band of 53 kDa in the treated cells. Show More Analysis of p53 acK382 in lymphocytes. Human peripheral blood mononuclear cells PBMC were either treated with 0.4 muM Trichostatin A Sigma, Cat. No. T8552 plus 0.4 muM Adriamycin Doxorubicin hydrochloride, Sigma, Cat. No. D1515 for 24 hours shaded histogram or untreated open histogram . The cells were fixed BD Cytofix buffer, Cat. No. 554655 for 10 minutes, then permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for at least 30 minutes, and then stained with Alexa Fluor 647 Mouse anti-p53 acK382 . Lymphocytes were selected by scatter profile. Flow cytometry was performed on a BD FACSArray bioanalyzer system. The specificity of mAb L82-51 was confirmed by western blot analysis using unconjugated antibody on lysates from control left blot and Trichostatin A-plus-Adriamycin-treated right blot PBMC. p53 acK382 is identified as a band of 53 kDa in the treated cells.