BD PhosflowTM Alexa Fluor 647 Mouse anti-PLCgamma1

Description

Analysis of PLCgamma1 in human T leukemia cell lines. Jurkat ATCC TIB-152, open histogram and J.gamma1 PLCgamma1-deficient Jurkat, ATCC CRL-2678, shaded histogram were fixed with pre-warmed BD Cytofix buffer Cat. No. 554655 for 10 minutes at 37oC, permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 on ice for at least 30 minutes, and then stained with Alexa Fluor 647 Mouse anti-PLCgamma1 Cat. No. 558565 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Analysis of PLCgamma1 in human T leukemia cell lines. Jurkat ATCC TIB-152, open histogram and J.gamma1 PLCgamma1-deficient Jurkat, ATCC CRL-2678, shaded histogram were fixed with pre-warmed BD Cytofix buffer Cat. No. 554655 for 10 minutes at 37oC, permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 on ice for at least 30 minutes, and then stained with Alexa Fluor 647 Mouse anti-PLCgamma1 Cat. No. 558565 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Show More Analysis of PLCgamma1 in human T leukemia cell lines. Jurkat ATCC TIB-152, open histogram and J.gamma1 PLCgamma1-deficient Jurkat, ATCC CRL-2678, shaded histogram were fixed with pre-warmed BD Cytofix buffer Cat. No. 554655 for 10 minutes at 37oC, permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 on ice for at least 30 minutes, and then stained with Alexa Fluor 647 Mouse anti-PLCgamma1 Cat. No. 558565 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system.