BD PhosflowTM Alexa Fluor 647 Mouse anti-PTEN

Description

Analysis of PTEN in human epithelioid carcinoma. HeLa S3 cells ATCC CCL 2.2 were either transfected with PTEN RNAi open histogram or untreated shaded histogram . The cells were fixed using BD Cytofix buffer Cat. No. 554655 for 10 minutes at 37 C, then permeabilized usingBD Phosflow Perm Buffer III Cat. No. 558050 on ice for 30 minutes, and then stained with Alexa Fluor 647 Mouse anti-PTEN. Down-regulation of PTEN expression is evident in the RNAi-transfected cells. Flow cytometry was performed on a BD FACSArray bioanalyzer system. Analysis of PTEN in human epithelioid carcinoma. HeLa S3 cells ATCC CCL 2.2 were either transfected with PTEN RNAi open histogram or untreated shaded histogram . The cells were fixed using BD Cytofix buffer Cat. No. 554655 for 10 minutes at 37 C, then permeabilized usingBD Phosflow Perm Buffer III Cat. No. 558050 on ice for 30 minutes, and then stained with Alexa Fluor 647 Mouse anti-PTEN. Down-regulation of PTEN expression is evident in the RNAi-transfected cells. Flow cytometry was performed on a BD FACSArray bioanalyzer system. Show More Analysis of PTEN in human epithelioid carcinoma. HeLa S3 cells ATCC CCL 2.2 were either transfected with PTEN RNAi open histogram or untreated shaded histogram . The cells were fixed using BD Cytofix buffer Cat. No. 554655 for 10 minutes at 37 C, then permeabilized usingBD Phosflow Perm Buffer III Cat. No. 558050 on ice for 30 minutes, and then stained with Alexa Fluor 647 Mouse anti-PTEN. Down-regulation of PTEN expression is evident in the RNAi-transfected cells. Flow cytometry was performed on a BD FACSArray bioanalyzer system.