BD PhosflowTM Alexa Fluor 647 Mouse anti-Total Stat1 (N-Terminus)

Description

Analysis of Stat1 in human epithelioid carcinoma. HeLa S3 cells ATCC CCL 2.2 were either transfected with Stat1 RNAi open histogram or untreated shaded histogram . The cells were fixed BD Cytofix buffer, Cat. No. 554655 for 10 minutes at 37 C, then permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for 30 minutes, and then stained with Alexa Fluor 647 Mouse anti-Total Stat1 N-Terminus . Down-regulation of Stat1 expression is evident in the RNAi-transfected cells. Flow cytometry was performed on a BD FACSArray bioanalyzer system. Analysis of Stat1 in human epithelioid carcinoma. HeLa S3 cells ATCC CCL 2.2 were either transfected with Stat1 RNAi open histogram or untreated shaded histogram . The cells were fixed BD Cytofix buffer, Cat. No. 554655 for 10 minutes at 37 C, then permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for 30 minutes, and then stained with Alexa Fluor 647 Mouse anti-Total Stat1 N-Terminus . Down-regulation of Stat1 expression is evident in the RNAi-transfected cells. Flow cytometry was performed on a BD FACSArray bioanalyzer system. Show More Analysis of Stat1 in human epithelioid carcinoma. HeLa S3 cells ATCC CCL 2.2 were either transfected with Stat1 RNAi open histogram or untreated shaded histogram . The cells were fixed BD Cytofix buffer, Cat. No. 554655 for 10 minutes at 37 C, then permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for 30 minutes, and then stained with Alexa Fluor 647 Mouse anti-Total Stat1 N-Terminus . Down-regulation of Stat1 expression is evident in the RNAi-transfected cells. Flow cytometry was performed on a BD FACSArray bioanalyzer system.