BD PhosflowTM APC Mouse anti-Stat3

Description

Analysis of Stat3 in human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were fixed BD Cytofix buffer, Cat. No. 554655 for 10 minutes at 37oC, then permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for 30 minutes, and then stained with either APC Mouse IgG1, kappa Isotype Control Cat. No. 554681, shaded histogram or APC Mouse anti-Stat3 Cat. No. 560392, open histogram . For data analysis, lymphocytes were selected by their scatter profile. Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Analysis of Stat3 in human epithelioid carcinoma. HeLa S3 cells ATCC CCL 2.2 were either transfected with Stat3 RNAi shaded histogram or untreated open histogram . The cells were fixed BD Cytofix buffer, Cat. No. 554655 for 10 minutes at 37C, then permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for 30 minutes, and then stained with APC Mouse anti-Stat3. Flow cytometry was performed on a BD FACSArray bioanalyzer system. Analysis of Stat3 in human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were fixed BD Cytofix buffer, Cat. No. 554655 for 10 minutes at 37oC, then permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for 30 minutes, and then stained with either APC Mouse IgG1, kappa Isotype Control Cat. No. 554681, shaded histogram or APC Mouse anti-Stat3 Cat. No. 560392, open histogram . For data analysis, lymphocytes were selected by their scatter profile. Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Show More Analysis of Stat3 in human epithelioid carcinoma. HeLa S3 cells ATCC CCL 2.2 were either transfected with Stat3 RNAi shaded histogram or untreated open histogram . The cells were fixed BD Cytofix buffer, Cat. No. 554655 for 10 minutes at 37C, then permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for 30 minutes, and then stained with APC Mouse anti-Stat3. Flow cytometry was performed on a BD FACSArray bioanalyzer system. Show More Analysis of Stat3 in human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were fixed BD Cytofix buffer, Cat. No. 554655 for 10 minutes at 37oC, then permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for 30 minutes, and then stained with either APC Mouse IgG1, kappa Isotype Control Cat. No. 554681, shaded histogram or APC Mouse anti-Stat3 Cat. No. 560392, open histogram . For data analysis, lymphocytes were selected by their scatter profile. Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Analysis of Stat3 in human epithelioid carcinoma. HeLa S3 cells ATCC CCL 2.2 were either transfected with Stat3 RNAi shaded histogram or untreated open histogram . The cells were fixed BD Cytofix buffer, Cat. No. 554655 for 10 minutes at 37C, then permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for 30 minutes, and then stained with APC Mouse anti-Stat3. Flow cytometry was performed on a BD FACSArray bioanalyzer system.