BD PhosflowTM BV421 Mouse Anti-Btk (pY223)/Itk (pY180)

Description

Analyses of BTK pY223 /ITK pY180 expression by Human and Mouse Cells. Left Panel - Flow cytometric analysis of BTK pY223 expressed by human Ramos cells. Serum-starved cells from the human Ramos Burkitts lymphoma, ATCC CRL-1596 cell line were either not stimulated dashed line histogram or stimulated solid line histogram with Goat F ab 2 Anti-Human IgM Southern Biotech, Cat. No. 2022-14 at 37C for 3 minutes. Cells were fixed in BD Cytofix Buffer Cat. No. 554655; 37C for 10 min and permeabilized in BD Phosflow Perm Buffer III Cat. No. 558050 on ice 30 min . Cells were then stained with BD Phosflow BV421 Mouse Anti-Btk pY223 /Itk pY180 antibody Cat. No. 564848 . Histograms showing BTK pY223 expression were derived from gated events with the forward and side light-scatter characteristics of intact cells using a BD LSR II Flow Cytometer System. Right Panel - Flow cytometric analysis of BTK pY223 expressed by mouse A20 cells. Cells from the mouse A20 B lymphoma, ATCC TIB-208 cell line were either not stimulated dashed line histogram or were stimulated solid line histogram with Rabbit Anti-Mouse IgM H+L Jackson, Cat. No. 315-005-003 at 37 C for 3 minutes. The cells were fixed, permeabilized, stained, and analyzed for BTK pY223 by flow cytometry as described above. Analyses of BTK pY223 /ITK pY180 expression by Human and Mouse Cells. Left Panel - Flow cytometric analysis of BTK pY223 expressed by human Ramos cells. Serum-starved cells from the human Ramos Burkitts lymphoma, ATCC CRL-1596 cell line were either not stimulated dashed line histogram or stimulated solid line histogram with Goat F ab 2 Anti-Human IgM Southern Biotech, Cat. No. 2022-14 at 37C for 3 minutes. Cells were fixed in BD Cytofix Buffer Cat. No. 554655; 37C for 10 min and permeabilized in BD Phosflow Perm Buffer III Cat. No. 558050 on ice 30 min . Cells were then stained with BD Phosflow BV421 Mouse Anti-Btk pY223 /Itk pY180 antibody Cat. No. 564848 . Histograms showing BTK pY223 expression were derived from gated events with the forward and side light-scatter characteristics of intact cells using a BD LSR II Flow Cytometer System. Right Panel - Flow cytometric analysis of BTK pY223 expressed by mouse A20 cells. Cells from the mouse A20 B lymphoma, ATCC TIB-208 cell line were either not stimulated dashed line histogram or were stimulated solid line histogram with Rabbit Anti-Mouse IgM H+L Jackson, Cat. No. 315-005-003 at 37 C for 3 minutes. The cells were fixed, permeabilized, stained, and analyzed for BTK pY223 by flow cytometry as described above. Analyses of BTK pY223 /ITK pY180 expression by Human and Mouse Cells. Left Panel - Flow cytometric analysis of BTK pY223 expressed by human Ramos cells. Serum-starved cells from the human Ramos Burkitts lymphoma, ATCC CRL-1596 cell line were either not stimulated dashed line histogram or stimulated solid line histogram with Goat F ab 2 Anti-Human IgM Southern Biotech, Cat. No. 2022-14 at 37C for 3 minutes. Cells were fixed in BD Cytofix Buffer Cat. No. 554655; 37C for 10 min and permeabilized in BD Phosflow Perm Buffer III Cat. No. 558050 on ice 30 min . Cells were then stained with BD Phosflow BV421 Mouse Anti-Btk pY223 /Itk pY180 antibody Cat. No. 564848 . Histograms showing BTK pY223 expression were derived from gated events with the forward and side light-scatter characteristics of intact cells using a BD LSR II Flow Cytometer System. Right Panel - Flow cytometric analysis of BTK pY223 expressed by mouse A20 cells. Cells from the mouse A20 B lymphoma, ATCC TIB-208 cell line were either not stimulated dashed line histogram or were stimulated solid line histogram with Rabbit Anti-Mouse IgM H+L Jackson, Cat. No. 315-005-003 at 37 C for 3 minutes. The cells were fixed, permeabilized, stained, and analyzed for BTK pY223 by flow cytometry as described above. Show More Analyses of BTK pY223 /ITK pY180 expression by Human and Mouse Cells. Left Panel - Flow cytometric analysis of BTK pY223 expressed by human Ramos cells. Serum-starved cells from the human Ramos Burkitts lymphoma, ATCC CRL-1596 cell line were either not stimulated dashed line histogram or stimulated solid line histogram with Goat F ab 2 Anti-Human IgM Southern Biotech, Cat. No. 2022-14 at 37C for 3 minutes. Cells were fixed in BD Cytofix Buffer Cat. No. 554655; 37C for 10 min and permeabilized in BD Phosflow Perm Buffer III Cat. No. 558050 on ice 30 min . Cells were then stained with BD Phosflow BV421 Mouse Anti-Btk pY223 /Itk pY180 antibody Cat. No. 564848 . Histograms showing BTK pY223 expression were derived from gated events with the forward and side light-scatter characteristics of intact cells using a BD LSR II Flow Cytometer System. Right Panel - Flow cytometric analysis of BTK pY223 expressed by mouse A20 cells. Cells from the mouse A20 B lymphoma, ATCC TIB-208 cell line were either not stimulated dashed line histogram or were stimulated solid line histogram with Rabbit Anti-Mouse IgM H+L Jackson, Cat. No. 315-005-003 at 37 C for 3 minutes. The cells were fixed, permeabilized, stained, and analyzed for BTK pY223 by flow cytometry as described above. Show More Analyses of BTK pY223 /ITK pY180 expression by Human and Mouse Cells. Left Panel - Flow cytometric analysis of BTK pY223 expressed by human Ramos cells. Serum-starved cells from the human Ramos Burkitts lymphoma, ATCC CRL-1596 cell line were either not stimulated dashed line histogram or stimulated solid line histogram with Goat F ab 2 Anti-Human IgM Southern Biotech, Cat. No. 2022-14 at 37C for 3 minutes. Cells were fixed in BD Cytofix Buffer Cat. No. 554655; 37C for 10 min and permeabilized in BD Phosflow Perm Buffer III Cat. No. 558050 on ice 30 min . Cells were then stained with BD Phosflow BV421 Mouse Anti-Btk pY223 /Itk pY180 antibody Cat. No. 564848 . Histograms showing BTK pY223 expression were derived from gated events with the forward and side light-scatter characteristics of intact cells using a BD LSR II Flow Cytometer System. Right Panel - Flow cytometric analysis of BTK pY223 expressed by mouse A20 cells. Cells from the mouse A20 B lymphoma, ATCC TIB-208 cell line were either not stimulated dashed line histogram or were stimulated solid line histogram with Rabbit Anti-Mouse IgM H+L Jackson, Cat. No. 315-005-003 at 37 C for 3 minutes. The cells were fixed, permeabilized, stained, and analyzed for BTK pY223 by flow cytometry as described above. Analyses of BTK pY223 /ITK pY180 expression by Human and Mouse Cells. Left Panel - Flow cytometric analysis of BTK pY223 expressed by human Ramos cells. Serum-starved cells from the human Ramos Burkitts lymphoma, ATCC CRL-1596 cell line were either not stimulated dashed line histogram or stimulated solid line histogram with Goat F ab 2 Anti-Human IgM Southern Biotech, Cat. No. 2022-14 at 37C for 3 minutes. Cells were fixed in BD Cytofix Buffer Cat. No. 554655; 37C for 10 min and permeabilized in BD Phosflow Perm Buffer III Cat. No. 558050 on ice 30 min . Cells were then stained with BD Phosflow BV421 Mouse Anti-Btk pY223 /Itk pY180 antibody Cat. No. 564848 . Histograms showing BTK pY223 expression were derived from gated events with the forward and side light-scatter characteristics of intact cells using a BD LSR II Flow Cytometer System. Right Panel - Flow cytometric analysis of BTK pY223 expressed by mouse A20 cells. Cells from the mouse A20 B lymphoma, ATCC TIB-208 cell line were either not stimulated dashed line histogram or were stimulated solid line histogram with Rabbit Anti-Mouse IgM H+L Jackson, Cat. No. 315-005-003 at 37 C for 3 minutes. The cells were fixed, permeabilized, stained, and analyzed for BTK pY223 by flow cytometry as described above.