BD PhosflowTM BV421 Mouse Anti-ERK1/2 (pT202/pY204)

Description

Flow cytometric analysis of Erk1/2 pT202/pY204 expression in stimulated human peripheral blood lymphocytes. Whole blood was either left untreated dashed line histogram or treated solid line histogram with 400 nM Phorbol 12-Myristate 13-Acetate PMA; Sigma, Cat. No. P8139 for 15 minutes at 37C. Erythrocytes were lysed and the leucocytes were fixed with 1X BD Phosflow Lyse/Fix Buffer Cat. No. 558049 for 10 minutes at 37C. The cells were then permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 on ice for 30 minutes and stained with BD Phosflow BV421 Mouse Anti-Erk1/2 pT202/pY204 Cat No. 562981 . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry was performed using a BD LSRFortessa Cell Analyzer System. Flow cytometric analysis of Erk1/2 pT202/pY204 expression in stimulated human peripheral blood lymphocytes. Whole blood was either left untreated dashed line histogram or treated solid line histogram with 400 nM Phorbol 12-Myristate 13-Acetate PMA; Sigma, Cat. No. P8139 for 15 minutes at 37C. Erythrocytes were lysed and the leucocytes were fixed with 1X BD Phosflow Lyse/Fix Buffer Cat. No. 558049 for 10 minutes at 37C. The cells were then permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 on ice for 30 minutes and stained with BD Phosflow BV421 Mouse Anti-Erk1/2 pT202/pY204 Cat No. 562981 . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry was performed using a BD LSRFortessa Cell Analyzer System. Show More Flow cytometric analysis of Erk1/2 pT202/pY204 expression in stimulated human peripheral blood lymphocytes. Whole blood was either left untreated dashed line histogram or treated solid line histogram with 400 nM Phorbol 12-Myristate 13-Acetate PMA; Sigma, Cat. No. P8139 for 15 minutes at 37C. Erythrocytes were lysed and the leucocytes were fixed with 1X BD Phosflow Lyse/Fix Buffer Cat. No. 558049 for 10 minutes at 37C. The cells were then permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 on ice for 30 minutes and stained with BD Phosflow BV421 Mouse Anti-Erk1/2 pT202/pY204 Cat No. 562981 . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry was performed using a BD LSRFortessa Cell Analyzer System.