BD PhosflowTM BV421 Mouse Anti-Human CD20

Description

Flow cytometric analysis of CD20 cytoplasmic domain expression by human peripheral blood lymphocytes. Human whole blood was treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 for 10 min at 37C to lyse erythrocytes and fix the leucocytes in one step. The leucocytes were permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 for 20 minutes. The cells were then stained with either BD Horizon BV421 Mouse IgG2a, kappa Isotype Control Cat. No. 563464; dashed line histogram or BD Phosflow BV421 Mouse Anti-Human CD20 cytoplasmic antibody Cat. No. 563346; solid line histogram . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Flow cytometric analysis of CD20 cytoplasmic domain expression by human peripheral blood lymphocytes. Human whole blood was treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 for 10 min at 37C to lyse erythrocytes and fix the leucocytes in one step. The leucocytes were permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 for 20 minutes. The cells were then stained with either BD Horizon BV421 Mouse IgG2a, kappa Isotype Control Cat. No. 563464; dashed line histogram or BD Phosflow BV421 Mouse Anti-Human CD20 cytoplasmic antibody Cat. No. 563346; solid line histogram . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Flow cytometric analysis of CD20 cytoplasmic domain expression by human peripheral blood lymphocytes. Human whole blood was treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 for 10 min at 37C to lyse erythrocytes and fix the leucocytes in one step. The leucocytes were permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 for 20 minutes. The cells were then stained with either BD Horizon BV421 Mouse IgG2a, kappa Isotype Control Cat. No. 563464; dashed line histogram or BD Phosflow BV421 Mouse Anti-Human CD20 cytoplasmic antibody Cat. No. 563346; solid line histogram . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System.