BD PhosflowTM BV421 Mouse Anti-Stat1 (pY701)

Description

Flow cytometric analysis of Stat1 pY701 expression in human peripheral blood monocytes. Peripheral blood mononuclear cells PBMC were either left unstimulated dashed line histogram or stimulated solid line histogram with 100 ng/ml final concentration of Recombinant Human IFN-gamma Cat. No. 554617 for 15 minutes at 37C. The PBMC were fixed 10 min, 37C with BD Cytofix Fixation Buffer Cat. No. 554655 and then permeabilized on ice, 30 min with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and stained with BD Phosflow BV421 Mouse Anti-Stat1 pY701 antibody Cat. No. 562985 . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact monocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Flow cytometric analysis of Stat1 pY701 expression in human peripheral blood monocytes. Peripheral blood mononuclear cells PBMC were either left unstimulated dashed line histogram or stimulated solid line histogram with 100 ng/ml final concentration of Recombinant Human IFN-gamma Cat. No. 554617 for 15 minutes at 37C. The PBMC were fixed 10 min, 37C with BD Cytofix Fixation Buffer Cat. No. 554655 and then permeabilized on ice, 30 min with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and stained with BD Phosflow BV421 Mouse Anti-Stat1 pY701 antibody Cat. No. 562985 . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact monocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Flow cytometric analysis of Stat1 pY701 expression in human peripheral blood monocytes. Peripheral blood mononuclear cells PBMC were either left unstimulated dashed line histogram or stimulated solid line histogram with 100 ng/ml final concentration of Recombinant Human IFN-gamma Cat. No. 554617 for 15 minutes at 37C. The PBMC were fixed 10 min, 37C with BD Cytofix Fixation Buffer Cat. No. 554655 and then permeabilized on ice, 30 min with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and stained with BD Phosflow BV421 Mouse Anti-Stat1 pY701 antibody Cat. No. 562985 . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact monocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Flow cytometric analysis of Stat1 pY701 expression in human peripheral blood monocytes. Peripheral blood mononuclear cells PBMC were either left unstimulated dashed line histogram or stimulated solid line histogram with 100 ng/ml final concentration of Recombinant Human IFN-gamma Cat. No. 554617 for 15 minutes at 37C. The PBMC were fixed 10 min, 37C with BD Cytofix Fixation Buffer Cat. No. 554655 and then permeabilized on ice, 30 min with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and stained with BD Phosflow BV421 Mouse Anti-Stat1 pY701 antibody Cat. No. 562985 . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact monocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Flow cytometric analysis of Stat1 pY701 expression in human peripheral blood monocytes. Peripheral blood mononuclear cells PBMC were either left unstimulated dashed line histogram or stimulated solid line histogram with 100 ng/ml final concentration of Recombinant Human IFN-gamma Cat. No. 554617 for 15 minutes at 37C. The PBMC were fixed 10 min, 37C with BD Cytofix Fixation Buffer Cat. No. 554655 and then permeabilized on ice, 30 min with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and stained with BD Phosflow BV421 Mouse Anti-Stat1 pY701 antibody Cat. No. 562985 . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact monocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Flow cytometric analysis of Stat1 pY701 expression in human peripheral blood monocytes. Peripheral blood mononuclear cells PBMC were either left unstimulated dashed line histogram or stimulated solid line histogram with 100 ng/ml final concentration of Recombinant Human IFN-gamma Cat. No. 554617 for 15 minutes at 37C. The PBMC were fixed 10 min, 37C with BD Cytofix Fixation Buffer Cat. No. 554655 and then permeabilized on ice, 30 min with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and stained with BD Phosflow BV421 Mouse Anti-Stat1 pY701 antibody Cat. No. 562985 . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact monocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System.