BD PhosflowTM BV421 Mouse Anti-Stat3 (pS727)

Description

Flow cytometric analysis of Stat3 pS727 expression in human monocytes. Human peripheral blood mononuclear cells PBMC were either left untreated dashed line histogram or stimulated solid line histogram with 40 nM PMA Phorbol 12-Myristate 13-Acetate; Sigma Cat. No. P8139; 15 min at 37C . The cells were fixed 10 min with BD Cytofix Fixation Buffer Cat. No. 554655 , and permeabilized 30 min with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were then stained with BD Phosflow BV421 Mouse Anti-Stat3 pS727 antibody Cat. No. 565416 . The fluorescence histograms showing Stat3 pS727 expression were derived from gated events with the forward and side light-scatter characteristics of intact monocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Flow cytometric analysis of Stat3 pS727 expression in human monocytes. Human peripheral blood mononuclear cells PBMC were either left untreated dashed line histogram or stimulated solid line histogram with 40 nM PMA Phorbol 12-Myristate 13-Acetate; Sigma Cat. No. P8139; 15 min at 37C . The cells were fixed 10 min with BD Cytofix Fixation Buffer Cat. No. 554655 , and permeabilized 30 min with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were then stained with BD Phosflow BV421 Mouse Anti-Stat3 pS727 antibody Cat. No. 565416 . The fluorescence histograms showing Stat3 pS727 expression were derived from gated events with the forward and side light-scatter characteristics of intact monocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Flow cytometric analysis of Stat3 pS727 expression in human monocytes. Human peripheral blood mononuclear cells PBMC were either left untreated dashed line histogram or stimulated solid line histogram with 40 nM PMA Phorbol 12-Myristate 13-Acetate; Sigma Cat. No. P8139; 15 min at 37C . The cells were fixed 10 min with BD Cytofix Fixation Buffer Cat. No. 554655 , and permeabilized 30 min with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were then stained with BD Phosflow BV421 Mouse Anti-Stat3 pS727 antibody Cat. No. 565416 . The fluorescence histograms showing Stat3 pS727 expression were derived from gated events with the forward and side light-scatter characteristics of intact monocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System.