BD PhosflowTM Lyse/Fix Buffer 5X

Description

Flow cytometric analysis of ERK1/2 pT202/pY204 on human whole blood treated with BD Phosflow Lyse/Fix Buffer. Freshly isolated human whole blood was left either untreated shaded histogram or treated with 400 nM PMA at 37C for 10 minutes open histogram , then fixed with Lyse/Fix Buffer 5X Cat. No. 558049 . Leukocytes were spun down and washed once with Hanks solution, followed by permeabilization with BD Phosflow Perm Buffer II Cat. No. 558052 for 30 minutes. After washing the cells twice with BD Pharmingen Stain Buffer Cat. No. 554656554657 , the cells were stained with Alexa Fluor 488 mouse anti-ERK1/2 pT202/pY204 Cat. No. 612592 . Fluorescence histograms depicting ERK1/2 pT202/pY204 expression were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis of ERK1/2 pT202/pY204 on human whole blood treated with BD Phosflow Lyse/Fix Buffer. Freshly isolated human whole blood was left either untreated shaded histogram or treated with 400 nM PMA at 37C for 10 minutes open histogram , then fixed with Lyse/Fix Buffer 5X Cat. No. 558049 . Leukocytes were spun down and washed once with Hanks solution, followed by permeabilization with BD Phosflow Perm Buffer II Cat. No. 558052 for 30 minutes. After washing the cells twice with BD Pharmingen Stain Buffer Cat. No. 554656554657 , the cells were stained with Alexa Fluor 488 mouse anti-ERK1/2 pT202/pY204 Cat. No. 612592 . Fluorescence histograms depicting ERK1/2 pT202/pY204 expression were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Show More Flow cytometric analysis of ERK1/2 pT202/pY204 on human whole blood treated with BD Phosflow Lyse/Fix Buffer. Freshly isolated human whole blood was left either untreated shaded histogram or treated with 400 nM PMA at 37C for 10 minutes open histogram , then fixed with Lyse/Fix Buffer 5X Cat. No. 558049 . Leukocytes were spun down and washed once with Hanks solution, followed by permeabilization with BD Phosflow Perm Buffer II Cat. No. 558052 for 30 minutes. After washing the cells twice with BD Pharmingen Stain Buffer Cat. No. 554656554657 , the cells were stained with Alexa Fluor 488 mouse anti-ERK1/2 pT202/pY204 Cat. No. 612592 . Fluorescence histograms depicting ERK1/2 pT202/pY204 expression were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.