BD PhosflowTM PE Mouse anti-Akt (pS473)

Description

LEFT: Analysis of Akt pS473 in mouse embryonic fibroblasts. Serum-starved NIH/3T3 cells ATCC CRL-1658 were either stimulated with PDGF-BB Cat. No. 354051, open histogram or unstimulated shaded histogram . The cells were fixed BD Cytofix Fixation Buffer, Cat. No. 554655 for 10 minutes at 37C, then permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for at least 30 minutes, and then stained with PE Mouse anti-Akt pS473 . Flow cytometry was performed on a BD FACSArray flow cytometry system. RIGHT: Analysis of Akt pS473 in human T-cell leukemia. Jurkat cells ATCC TIB-152 were either treated with 1 muM Wortmannin Life Technologies, Cat. No. PHZ1301 for 2 hours at 37C shaded histogram or left untreated open histogram . The cells were fixed BD Cytofix Fixation Buffer, Cat. No. 554655 for 10 minutes at 37C, then permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for at least 30 minutes, and then stained with PE Mouse anti-Akt pS473 . The data demonstrates that the level of phosphorylation of Akt pS473 decreases when phosphatidylinositol 3-kinase activity is inhibited by the treatment. Flow cytometry was performed on a BD FACSArray flow cytometry system. LEFT: Analysis of Akt pS473 in mouse embryonic fibroblasts. Serum-starved NIH/3T3 cells ATCC CRL-1658 were either stimulated with PDGF-BB Cat. No. 354051, open histogram or unstimulated shaded histogram . The cells were fixed BD Cytofix Fixation Buffer, Cat. No. 554655 for 10 minutes at 37C, then permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for at least 30 minutes, and then stained with PE Mouse anti-Akt pS473 . Flow cytometry was performed on a BD FACSArray flow cytometry system. RIGHT: Analysis of Akt pS473 in human T-cell leukemia. Jurkat cells ATCC TIB-152 were either treated with 1 muM Wortmannin Life Technologies, Cat. No. PHZ1301 for 2 hours at 37C shaded histogram or left untreated open histogram . The cells were fixed BD Cytofix Fixation Buffer, Cat. No. 554655 for 10 minutes at 37C, then permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for at least 30 minutes, and then stained with PE Mouse anti-Akt pS473 . The data demonstrates that the level of phosphorylation of Akt pS473 decreases when phosphatidylinositol 3-kinase activity is inhibited by the treatment. Flow cytometry was performed on a BD FACSArray flow cytometry system. Show More LEFT: Analysis of Akt pS473 in mouse embryonic fibroblasts. Serum-starved NIH/3T3 cells ATCC CRL-1658 were either stimulated with PDGF-BB Cat. No. 354051, open histogram or unstimulated shaded histogram . The cells were fixed BD Cytofix Fixation Buffer, Cat. No. 554655 for 10 minutes at 37C, then permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for at least 30 minutes, and then stained with PE Mouse anti-Akt pS473 . Flow cytometry was performed on a BD FACSArray flow cytometry system. RIGHT: Analysis of Akt pS473 in human T-cell leukemia. Jurkat cells ATCC TIB-152 were either treated with 1 muM Wortmannin Life Technologies, Cat. No. PHZ1301 for 2 hours at 37C shaded histogram or left untreated open histogram . The cells were fixed BD Cytofix Fixation Buffer, Cat. No. 554655 for 10 minutes at 37C, then permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for at least 30 minutes, and then stained with PE Mouse anti-Akt pS473 . The data demonstrates that the level of phosphorylation of Akt pS473 decreases when phosphatidylinositol 3-kinase activity is inhibited by the treatment. Flow cytometry was performed on a BD FACSArray flow cytometry system.