BD PhosflowTM PE Mouse anti-CD247 (pY142)

Description

Analysis of CD247 CD3zeta pY142 in activated human T leukemia cells. Jurkat cells ATCC TIB-152 were either stimulated by cross-linking of CD3 and CD28 with NA/LE Mouse anti-Human CD3 mAb UCHT1 Cat. No. 555329 and NA/LE Mouse anti-Human CD28 mAb CD28.2 Cat. No. 555725 on ice for 15 minutes followed by Purified Goat anti-Mouse Ig Cat. No. 553998 on ice for 15 minutes, and then allowed to undergo phosphorylation at 37C for 2 minutes shaded histogram or unstimulated open histogram . The cells were fixed BD Phosflow Fix Buffer I, Cat. No. 557870 for 10-15 minutes at 37 C, permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for at least 30 minutes, blocked with normal mouse immunoglobulin, and then stained with PE Mouse anti-CD247 CD3zeta pY142 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Analysis of CD247 CD3zeta pY142 in activated human T leukemia cells. Jurkat cells ATCC TIB-152 were either stimulated by cross-linking of CD3 and CD28 with NA/LE Mouse anti-Human CD3 mAb UCHT1 Cat. No. 555329 and NA/LE Mouse anti-Human CD28 mAb CD28.2 Cat. No. 555725 on ice for 15 minutes followed by Purified Goat anti-Mouse Ig Cat. No. 553998 on ice for 15 minutes, and then allowed to undergo phosphorylation at 37C for 2 minutes shaded histogram or unstimulated open histogram . The cells were fixed BD Phosflow Fix Buffer I, Cat. No. 557870 for 10-15 minutes at 37 C, permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for at least 30 minutes, blocked with normal mouse immunoglobulin, and then stained with PE Mouse anti-CD247 CD3zeta pY142 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Show More Analysis of CD247 CD3zeta pY142 in activated human T leukemia cells. Jurkat cells ATCC TIB-152 were either stimulated by cross-linking of CD3 and CD28 with NA/LE Mouse anti-Human CD3 mAb UCHT1 Cat. No. 555329 and NA/LE Mouse anti-Human CD28 mAb CD28.2 Cat. No. 555725 on ice for 15 minutes followed by Purified Goat anti-Mouse Ig Cat. No. 553998 on ice for 15 minutes, and then allowed to undergo phosphorylation at 37C for 2 minutes shaded histogram or unstimulated open histogram . The cells were fixed BD Phosflow Fix Buffer I, Cat. No. 557870 for 10-15 minutes at 37 C, permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for at least 30 minutes, blocked with normal mouse immunoglobulin, and then stained with PE Mouse anti-CD247 CD3zeta pY142 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system.