BD PhosflowTM PE Mouse anti-elF4E (pS209)

Description

Analysis of eIF4E pS209 in monocytes. Human peripheral blood mononuclear cells PBMC were either stimulated with 40 nM Phorbol 12-myristate 13-acetate PMA, Sigma-Aldrich Cat. No. P8139 for 15 minutes shaded histogram or unstimulated open histogram . The cells were fixed BD Cytofix buffer, Cat. No. 554655 for 10 minutes, then permeabilized BD Phosflow Perm Buffer II, Cat. No. 558052 on ice for at least 30 minutes, and then stained with PE Mouse anti-elF4E pS209, Cat. No. 560229 . Monocytes were selected by scatter profile. Flow cytometry was performed on a BD FACSArray bioanalyzer system. The specificity of mAb J77-925 was confirmed by western blot analysis using unconjugated antibody on lysates from control lane 1 and PMA-treated lane 2 PBMC. elF4E pS209 is identified as a band of 25 kDa, with increased intensity in the treated lane. Analysis of eIF4E pS209 in monocytes. Human peripheral blood mononuclear cells PBMC were either stimulated with 40 nM Phorbol 12-myristate 13-acetate PMA, Sigma-Aldrich Cat. No. P8139 for 15 minutes shaded histogram or unstimulated open histogram . The cells were fixed BD Cytofix buffer, Cat. No. 554655 for 10 minutes, then permeabilized BD Phosflow Perm Buffer II, Cat. No. 558052 on ice for at least 30 minutes, and then stained with PE Mouse anti-elF4E pS209, Cat. No. 560229 . Monocytes were selected by scatter profile. Flow cytometry was performed on a BD FACSArray bioanalyzer system. The specificity of mAb J77-925 was confirmed by western blot analysis using unconjugated antibody on lysates from control lane 1 and PMA-treated lane 2 PBMC. elF4E pS209 is identified as a band of 25 kDa, with increased intensity in the treated lane. Show More Analysis of eIF4E pS209 in monocytes. Human peripheral blood mononuclear cells PBMC were either stimulated with 40 nM Phorbol 12-myristate 13-acetate PMA, Sigma-Aldrich Cat. No. P8139 for 15 minutes shaded histogram or unstimulated open histogram . The cells were fixed BD Cytofix buffer, Cat. No. 554655 for 10 minutes, then permeabilized BD Phosflow Perm Buffer II, Cat. No. 558052 on ice for at least 30 minutes, and then stained with PE Mouse anti-elF4E pS209, Cat. No. 560229 . Monocytes were selected by scatter profile. Flow cytometry was performed on a BD FACSArray bioanalyzer system. The specificity of mAb J77-925 was confirmed by western blot analysis using unconjugated antibody on lysates from control lane 1 and PMA-treated lane 2 PBMC. elF4E pS209 is identified as a band of 25 kDa, with increased intensity in the treated lane.