BD PhosflowTM PE Mouse anti-eNOS

Description

Analysis of eNOS in human endothelial cells. EA-hy 926 cells Edgell, McDonald, Graham, 1983 were either transfected with eNOS RNAi open histogram or untreated shaded histogram . The cells were fixed BD Cytofix Fixation buffer, Cat. No. 554655 for 10 minutes at 37C, then permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for 30 minutes, and then stained with PE Mouse anti-eNOS Cat. No. 560103 . Down-regulation of eNOS expression is evident in the RNAi-transfected cells. Flow cytometry was performed on a BD LSR II flow cytometry system. Analysis of eNOS in human endothelial cells. EA-hy 926 cells Edgell, McDonald, Graham, 1983 were either transfected with eNOS RNAi open histogram or untreated shaded histogram . The cells were fixed BD Cytofix Fixation buffer, Cat. No. 554655 for 10 minutes at 37C, then permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for 30 minutes, and then stained with PE Mouse anti-eNOS Cat. No. 560103 . Down-regulation of eNOS expression is evident in the RNAi-transfected cells. Flow cytometry was performed on a BD LSR II flow cytometry system. Show More Analysis of eNOS in human endothelial cells. EA-hy 926 cells Edgell, McDonald, Graham, 1983 were either transfected with eNOS RNAi open histogram or untreated shaded histogram . The cells were fixed BD Cytofix Fixation buffer, Cat. No. 554655 for 10 minutes at 37C, then permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for 30 minutes, and then stained with PE Mouse anti-eNOS Cat. No. 560103 . Down-regulation of eNOS expression is evident in the RNAi-transfected cells. Flow cytometry was performed on a BD LSR II flow cytometry system.