BD PhosflowTM PE Mouse Anti-ERK1/2 (pT202/pY204)

Description

Flow cytometric analysis of ERK1/2 pT202/pY204 . Human peripheral blood mononulcear cells PBMC were either left unstimulated unshaded or stimulated shaded with 40 nM PMA for 10 minutes at 37C. Cells were fixed with BD Cytofix buffer Cat. No. 554655 for 10 minutes at 37C and then permeabilized by adding BD Phosflow Perm Buffer III Cat. No. 558050 for 30 minutes on ice. Cells were then washed twice in BD Pharmingen Stain Buffer Cat. No. 554656 and stained with the PE mouse anti-ERK1/2 pT202/pY204 antibody. Cells were analyzed on a BD FACSCalibur flow cytometry instrument. For intracellular staining of human whole blood, BD Phosflow Lyse/Fix buffer Cat. No. 558049 may be used for fixation. Flow cytometric analysis of ERK1/2 pT202/pY204 . Human peripheral blood mononulcear cells PBMC were either left unstimulated unshaded or stimulated shaded with 40 nM PMA for 10 minutes at 37C. Cells were fixed with BD Cytofix buffer Cat. No. 554655 for 10 minutes at 37C and then permeabilized by adding BD Phosflow Perm Buffer III Cat. No. 558050 for 30 minutes on ice. Cells were then washed twice in BD Pharmingen Stain Buffer Cat. No. 554656 and stained with the PE mouse anti-ERK1/2 pT202/pY204 antibody. Cells were analyzed on a BD FACSCalibur flow cytometry instrument. For intracellular staining of human whole blood, BD Phosflow Lyse/Fix buffer Cat. No. 558049 may be used for fixation. Show More Flow cytometric analysis of ERK1/2 pT202/pY204 . Human peripheral blood mononulcear cells PBMC were either left unstimulated unshaded or stimulated shaded with 40 nM PMA for 10 minutes at 37C. Cells were fixed with BD Cytofix buffer Cat. No. 554655 for 10 minutes at 37C and then permeabilized by adding BD Phosflow Perm Buffer III Cat. No. 558050 for 30 minutes on ice. Cells were then washed twice in BD Pharmingen Stain Buffer Cat. No. 554656 and stained with the PE mouse anti-ERK1/2 pT202/pY204 antibody. Cells were analyzed on a BD FACSCalibur flow cytometry instrument. For intracellular staining of human whole blood, BD Phosflow Lyse/Fix buffer Cat. No. 558049 may be used for fixation.