BD PhosflowTM PE Mouse Anti-Human CD20

Description

Flow cytometric analysis of CD20 cytoplasmic domain expressed by human peripheral blood lymphocytes. Human whole blood was treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 for 10 min at 37C to lyse erythrocytes and fix the leukocytes in one step. The leukocytes were permeabilized with BD Phosflow Perm Buffer I Cat. No. 557885 for 20 minutes. The cells were then stained with either PE Mouse IgG2a, kappa Isotype Control MOPC-173 Cat. No. 558595; dashed line histogram or PE Mouse anti-human CD20 cytoplasmic antibody Cat. No. 561174; solid line histogram . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Flow cytometric analysis of CD20 cytoplasmic domain expressed by human peripheral blood lymphocytes. Human whole blood was treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 for 10 min at 37C to lyse erythrocytes and fix the leukocytes in one step. The leukocytes were permeabilized with BD Phosflow Perm Buffer I Cat. No. 557885 for 20 minutes. The cells were then stained with either PE Mouse IgG2a, kappa Isotype Control MOPC-173 Cat. No. 558595; dashed line histogram or PE Mouse anti-human CD20 cytoplasmic antibody Cat. No. 561174; solid line histogram . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Show More Flow cytometric analysis of CD20 cytoplasmic domain expressed by human peripheral blood lymphocytes. Human whole blood was treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 for 10 min at 37C to lyse erythrocytes and fix the leukocytes in one step. The leukocytes were permeabilized with BD Phosflow Perm Buffer I Cat. No. 557885 for 20 minutes. The cells were then stained with either PE Mouse IgG2a, kappa Isotype Control MOPC-173 Cat. No. 558595; dashed line histogram or PE Mouse anti-human CD20 cytoplasmic antibody Cat. No. 561174; solid line histogram . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System.