BD PhosflowTM PE Mouse Anti-Human Lck (pY505)

Description

Analysis of Lck pY505 in activated human T leukemia cells. Jurkat cells ATCC TIB-152 were serum starved overnight and then either stimulated with 5 mM hydrogen peroxide for 15 minutes shaded histogram or unstimulated open histogram . The cells were fixed with pre-warmed BD Cytofix buffer Cat. No. 554655 for 10 minutes, then permeabilized BD PhosFlow Perm Buffer III, Cat. No. 558050 on ice for at least 30 minutes, and then stained with PE anti-Lck pY505 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Analysis of Lck pY505 in activated human T leukemia cells. Jurkat cells ATCC TIB-152 were serum starved overnight and then either stimulated with 5 mM hydrogen peroxide for 15 minutes shaded histogram or unstimulated open histogram . The cells were fixed with pre-warmed BD Cytofix buffer Cat. No. 554655 for 10 minutes, then permeabilized BD PhosFlow Perm Buffer III, Cat. No. 558050 on ice for at least 30 minutes, and then stained with PE anti-Lck pY505 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Show More Analysis of Lck pY505 in activated human T leukemia cells. Jurkat cells ATCC TIB-152 were serum starved overnight and then either stimulated with 5 mM hydrogen peroxide for 15 minutes shaded histogram or unstimulated open histogram . The cells were fixed with pre-warmed BD Cytofix buffer Cat. No. 554655 for 10 minutes, then permeabilized BD PhosFlow Perm Buffer III, Cat. No. 558050 on ice for at least 30 minutes, and then stained with PE anti-Lck pY505 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system.