BD PhosflowTM PE Mouse anti-MAPKAPK-2 (pT334)

Description

Analyses of MAPKAPK2 pT334 expression by Human and Mouse Cells. Human Cells Panel 1a: Flow Cytometric analysis of MAPKAPK2 pT334 expressed by peripheral blood lymphocytes PBL . Whole blood cells were not stimulated dashed line histogram or stimulated solid line histogram with 400 nM Phorbol 12-Myristate 13-Acetate PMA; Sigma, Cat. No. P8139; 15 min, 37C . Cells were fixed in 1X BD Phosflow Lyse/Fix Buffer Cat. No. 558049; 10 min, 37 C and permeabilized in BD Phosflow Perm Buffer III Cat. No. 558050 on ice 30 min . Cells were then stained with BD Phosflow PE Mouse Anti-MAPKAPK2 pT334 Cat. No. 562470 antibody. Fluorescence histograms showing MAPKAPK2 pT334 expression were generated for gated events with the light scatter characteristics of intact lymphocytes using a BD FACSCanto II Flow Cytometer System. Panel 1b: Western blot analysis of MAPKAPK2 pT334 expressed by human peripheral blood mononuclear cells PBMC . Lysates from 1X106 untreated C and PMA-treated T PBMC were blotted using Purified Mouse Anti-MAPKAPK2 pT334 antibody 2.0 mug/ml, Cat. No. 562469 , HRP Goat Anti-Mouse Ig Cat. No. 554002 and a chemiluminescent detection system. MAPKAPK2 pT334 were identified as 49 kDa bands by Western blotting. Mouse Cells Panel 2: Splenocytes were not stimulated dashed line histogram or stimulated solid line histogram with PMA 50 nM, 15 min, 37C . Cells were fixed, permeabilized, stained with PE Mouse anti-MAPKAPK2 pT334 antibody and analyzed by flow cytometry as above. Analyses of MAPKAPK2 pT334 expression by Human and Mouse Cells. Human Cells Panel 1a: Flow Cytometric analysis of MAPKAPK2 pT334 expressed by peripheral blood lymphocytes PBL . Whole blood cells were not stimulated dashed line histogram or stimulated solid line histogram with 400 nM Phorbol 12-Myristate 13-Acetate PMA; Sigma, Cat. No. P8139; 15 min, 37C . Cells were fixed in 1X BD Phosflow Lyse/Fix Buffer Cat. No. 558049; 10 min, 37 C and permeabilized in BD Phosflow Perm Buffer III Cat. No. 558050 on ice 30 min . Cells were then stained with BD Phosflow PE Mouse Anti-MAPKAPK2 pT334 Cat. No. 562470 antibody. Fluorescence histograms showing MAPKAPK2 pT334 expression were generated for gated events with the light scatter characteristics of intact lymphocytes using a BD FACSCanto II Flow Cytometer System. Panel 1b: Western blot analysis of MAPKAPK2 pT334 expressed by human peripheral blood mononuclear cells PBMC . Lysates from 1X106 untreated C and PMA-treated T PBMC were blotted using Purified Mouse Anti-MAPKAPK2 pT334 antibody 2.0 mug/ml, Cat. No. 562469 , HRP Goat Anti-Mouse Ig Cat. No. 554002 and a chemiluminescent detection system. MAPKAPK2 pT334 were identified as 49 kDa bands by Western blotting. Mouse Cells Panel 2: Splenocytes were not stimulated dashed line histogram or stimulated solid line histogram with PMA 50 nM, 15 min, 37C . Cells were fixed, permeabilized, stained with PE Mouse anti-MAPKAPK2 pT334 antibody and analyzed by flow cytometry as above. Show More Analyses of MAPKAPK2 pT334 expression by Human and Mouse Cells. Human Cells Panel 1a: Flow Cytometric analysis of MAPKAPK2 pT334 expressed by peripheral blood lymphocytes PBL . Whole blood cells were not stimulated dashed line histogram or stimulated solid line histogram with 400 nM Phorbol 12-Myristate 13-Acetate PMA; Sigma, Cat. No. P8139; 15 min, 37C . Cells were fixed in 1X BD Phosflow Lyse/Fix Buffer Cat. No. 558049; 10 min, 37 C and permeabilized in BD Phosflow Perm Buffer III Cat. No. 558050 on ice 30 min . Cells were then stained with BD Phosflow PE Mouse Anti-MAPKAPK2 pT334 Cat. No. 562470 antibody. Fluorescence histograms showing MAPKAPK2 pT334 expression were generated for gated events with the light scatter characteristics of intact lymphocytes using a BD FACSCanto II Flow Cytometer System. Panel 1b: Western blot analysis of MAPKAPK2 pT334 expressed by human peripheral blood mononuclear cells PBMC . Lysates from 1X106 untreated C and PMA-treated T PBMC were blotted using Purified Mouse Anti-MAPKAPK2 pT334 antibody 2.0 mug/ml, Cat. No. 562469 , HRP Goat Anti-Mouse Ig Cat. No. 554002 and a chemiluminescent detection system. MAPKAPK2 pT334 were identified as 49 kDa bands by Western blotting. Mouse Cells Panel 2: Splenocytes were not stimulated dashed line histogram or stimulated solid line histogram with PMA 50 nM, 15 min, 37C . Cells were fixed, permeabilized, stained with PE Mouse anti-MAPKAPK2 pT334 antibody and analyzed by flow cytometry as above.