BD PhosflowTM PE Mouse anti-MEK1

Description

Analysis of MEK1 in HeLaS3 cells. HeLaS3 cells were either transfected with MEK1 RNAi open histogram or untreated shaded histogram . The cells were fixed BD Cytofix Fixation buffer, Cat. No. 554655 for 10 minutes at 37 C, then permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for 30 minutes, and then stained with PE Mouse anti-MEK1 Cat. No. 560099 . Down-regulation of MEK1 expression is evident in the RNAi-transfected cells. Flow cytometry was performed on a BD LSR II flow cytometry system. Analysis of MEK1 in HeLaS3 cells. HeLaS3 cells were either transfected with MEK1 RNAi open histogram or untreated shaded histogram . The cells were fixed BD Cytofix Fixation buffer, Cat. No. 554655 for 10 minutes at 37 C, then permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for 30 minutes, and then stained with PE Mouse anti-MEK1 Cat. No. 560099 . Down-regulation of MEK1 expression is evident in the RNAi-transfected cells. Flow cytometry was performed on a BD LSR II flow cytometry system. Show More Analysis of MEK1 in HeLaS3 cells. HeLaS3 cells were either transfected with MEK1 RNAi open histogram or untreated shaded histogram . The cells were fixed BD Cytofix Fixation buffer, Cat. No. 554655 for 10 minutes at 37 C, then permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for 30 minutes, and then stained with PE Mouse anti-MEK1 Cat. No. 560099 . Down-regulation of MEK1 expression is evident in the RNAi-transfected cells. Flow cytometry was performed on a BD LSR II flow cytometry system.