BD PhosflowTM PE Mouse anti-NF-kappaB p65 (pS529)

Description

Analysis of NFkB pS529 in human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were either stimulated with 50 nM PMA Sigma, P8139 for 15 minutes shaded histogram or unstimulated open histogram . The PBMC were fixed with BD Cytofix buffer Cat. No. 554655 for 10 minutes at 37C, permeabilized using BD Phosflow Perm Buffer III Cat. No. 558050 on ice for 30 minutes and then stained with PE Mouse anti-NF-kappaB p65 pS529 . For data analysis, lymphocytes were selected by scatter profile. Flow cytometry was performed on a BD FACSCanto II flow cytometry system. Analysis of NFkB pS529 in human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were either stimulated with 50 nM PMA Sigma, P8139 for 15 minutes shaded histogram or unstimulated open histogram . The PBMC were fixed with BD Cytofix buffer Cat. No. 554655 for 10 minutes at 37C, permeabilized using BD Phosflow Perm Buffer III Cat. No. 558050 on ice for 30 minutes and then stained with PE Mouse anti-NF-kappaB p65 pS529 . For data analysis, lymphocytes were selected by scatter profile. Flow cytometry was performed on a BD FACSCanto II flow cytometry system. Show More Analysis of NFkB pS529 in human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were either stimulated with 50 nM PMA Sigma, P8139 for 15 minutes shaded histogram or unstimulated open histogram . The PBMC were fixed with BD Cytofix buffer Cat. No. 554655 for 10 minutes at 37C, permeabilized using BD Phosflow Perm Buffer III Cat. No. 558050 on ice for 30 minutes and then stained with PE Mouse anti-NF-kappaB p65 pS529 . For data analysis, lymphocytes were selected by scatter profile. Flow cytometry was performed on a BD FACSCanto II flow cytometry system.