BD PhosflowTM PE Mouse anti-Rb (pS780)

Description

Analysis of Rb pS780 in activated human acute lymphoblastic leukemia. MOLT-4 cells without serum starvation were either stimulated with 200 ng/ml PMA Sigma, Cat. No. P8139 for 24 hours right panel or unstimulated left panel . The cells were fixed BD Phosflow Fix Buffer I, Cat. No. 557870 for 10 minutes at 37 C, then permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for at least 30 minutes, and then stained with PE Mouse anti-Rb pS780, Cat. No. 558548 . 7AAD staining solution Cat. No. 559925 was used to monitor the cell cycle of the stimulated and unstimulated cells. Flow cytometry was performed on a BD FACSCalibur flow cytometry system. The boxed region in the data demonstrates that PMA treatment causes cell cycle arrest at the G1 phase, which is associated with dephosphorylation of Rb. Analysis of Rb pS780 in activated human acute lymphoblastic leukemia. MOLT-4 cells without serum starvation were either stimulated with 200 ng/ml PMA Sigma, Cat. No. P8139 for 24 hours right panel or unstimulated left panel . The cells were fixed BD Phosflow Fix Buffer I, Cat. No. 557870 for 10 minutes at 37 C, then permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for at least 30 minutes, and then stained with PE Mouse anti-Rb pS780, Cat. No. 558548 . 7AAD staining solution Cat. No. 559925 was used to monitor the cell cycle of the stimulated and unstimulated cells. Flow cytometry was performed on a BD FACSCalibur flow cytometry system. The boxed region in the data demonstrates that PMA treatment causes cell cycle arrest at the G1 phase, which is associated with dephosphorylation of Rb. Analysis of Rb pS780 in activated human acute lymphoblastic leukemia. MOLT-4 cells without serum starvation were either stimulated with 200 ng/ml PMA Sigma, Cat. No. P8139 for 24 hours right panel or unstimulated left panel . The cells were fixed BD Phosflow Fix Buffer I, Cat. No. 557870 for 10 minutes at 37 C, then permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for at least 30 minutes, and then stained with PE Mouse anti-Rb pS780, Cat. No. 558548 . 7AAD staining solution Cat. No. 559925 was used to monitor the cell cycle of the stimulated and unstimulated cells. Flow cytometry was performed on a BD FACSCalibur flow cytometry system. The boxed region in the data demonstrates that PMA treatment causes cell cycle arrest at the G1 phase, which is associated with dephosphorylation of Rb. Show More Analysis of Rb pS780 in activated human acute lymphoblastic leukemia. MOLT-4 cells without serum starvation were either stimulated with 200 ng/ml PMA Sigma, Cat. No. P8139 for 24 hours right panel or unstimulated left panel . The cells were fixed BD Phosflow Fix Buffer I, Cat. No. 557870 for 10 minutes at 37 C, then permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for at least 30 minutes, and then stained with PE Mouse anti-Rb pS780, Cat. No. 558548 . 7AAD staining solution Cat. No. 559925 was used to monitor the cell cycle of the stimulated and unstimulated cells. Flow cytometry was performed on a BD FACSCalibur flow cytometry system. The boxed region in the data demonstrates that PMA treatment causes cell cycle arrest at the G1 phase, which is associated with dephosphorylation of Rb. Show More Analysis of Rb pS780 in activated human acute lymphoblastic leukemia. MOLT-4 cells without serum starvation were either stimulated with 200 ng/ml PMA Sigma, Cat. No. P8139 for 24 hours right panel or unstimulated left panel . The cells were fixed BD Phosflow Fix Buffer I, Cat. No. 557870 for 10 minutes at 37 C, then permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for at least 30 minutes, and then stained with PE Mouse anti-Rb pS780, Cat. No. 558548 . 7AAD staining solution Cat. No. 559925 was used to monitor the cell cycle of the stimulated and unstimulated cells. Flow cytometry was performed on a BD FACSCalibur flow cytometry system. The boxed region in the data demonstrates that PMA treatment causes cell cycle arrest at the G1 phase, which is associated with dephosphorylation of Rb. Analysis of Rb pS780 in activated human acute lymphoblastic leukemia. MOLT-4 cells without serum starvation were either stimulated with 200 ng/ml PMA Sigma, Cat. No. P8139 for 24 hours right panel or unstimulated left panel . The cells were fixed BD Phosflow Fix Buffer I, Cat. No. 557870 for 10 minutes at 37 C, then permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for at least 30 minutes, and then stained with PE Mouse anti-Rb pS780, Cat. No. 558548 . 7AAD staining solution Cat. No. 559925 was used to monitor the cell cycle of the stimulated and unstimulated cells. Flow cytometry was performed on a BD FACSCalibur flow cytometry system. The boxed region in the data demonstrates that PMA treatment causes cell cycle arrest at the G1 phase, which is associated with dephosphorylation of Rb.