BD PhosflowTM PE Mouse anti-SLP-76

Description

Analysis of SLP-76 in human peripheral blood lymphocytes. Human whole blood was lysed and fixed with 1X BD Phosflow Lyse/Fix Buffer Cat. No. 558049 for 10-15 minutes at 37oC, permeabilized BD Phosflow Perm Buffer II, Cat. No. 558052 on ice for 30 minutes, stained with Alexa Fluor 647 Mouse Anti-Human CD3 mAb UCHT1 Cat. No. 557706 , PerCP-Cy5.5 anti-human CD20 mAb H1 FB1 Cat. No. 558021 , and either PE Mouse anti-SLP-76 Cat. No. 560058, solid-line histograms or PE Mouse IgG2a, kappa Isotype control mAb MOPC-173 Cat. No. 558595, dashed-line histograms . The figures show lymphocyte subpopulations that were selected by their scatter profile and surface antigen expression. SLP-76 expression on CD3-positive CD20-negative T lymphocytes left panel , CD20-positive CD3-negative B lymphocytes center panel , and CD20-negative CD3-negative NK cells right panel are displayed. There was no detectable expression of SLP-76 in the monocyte/granulocyte population not shown . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Analysis of SLP-76 in human peripheral blood lymphocytes. Human whole blood was lysed and fixed with 1X BD Phosflow Lyse/Fix Buffer Cat. No. 558049 for 10-15 minutes at 37oC, permeabilized BD Phosflow Perm Buffer II, Cat. No. 558052 on ice for 30 minutes, stained with Alexa Fluor 647 Mouse Anti-Human CD3 mAb UCHT1 Cat. No. 557706 , PerCP-Cy5.5 anti-human CD20 mAb H1 FB1 Cat. No. 558021 , and either PE Mouse anti-SLP-76 Cat. No. 560058, solid-line histograms or PE Mouse IgG2a, kappa Isotype control mAb MOPC-173 Cat. No. 558595, dashed-line histograms . The figures show lymphocyte subpopulations that were selected by their scatter profile and surface antigen expression. SLP-76 expression on CD3-positive CD20-negative T lymphocytes left panel , CD20-positive CD3-negative B lymphocytes center panel , and CD20-negative CD3-negative NK cells right panel are displayed. There was no detectable expression of SLP-76 in the monocyte/granulocyte population not shown . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Analysis of SLP-76 in human peripheral blood lymphocytes. Human whole blood was lysed and fixed with 1X BD Phosflow Lyse/Fix Buffer Cat. No. 558049 for 10-15 minutes at 37oC, permeabilized BD Phosflow Perm Buffer II, Cat. No. 558052 on ice for 30 minutes, stained with Alexa Fluor 647 Mouse Anti-Human CD3 mAb UCHT1 Cat. No. 557706 , PerCP-Cy5.5 anti-human CD20 mAb H1 FB1 Cat. No. 558021 , and either PE Mouse anti-SLP-76 Cat. No. 560058, solid-line histograms or PE Mouse IgG2a, kappa Isotype control mAb MOPC-173 Cat. No. 558595, dashed-line histograms . The figures show lymphocyte subpopulations that were selected by their scatter profile and surface antigen expression. SLP-76 expression on CD3-positive CD20-negative T lymphocytes left panel , CD20-positive CD3-negative B lymphocytes center panel , and CD20-negative CD3-negative NK cells right panel are displayed. There was no detectable expression of SLP-76 in the monocyte/granulocyte population not shown . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Show More Analysis of SLP-76 in human peripheral blood lymphocytes. Human whole blood was lysed and fixed with 1X BD Phosflow Lyse/Fix Buffer Cat. No. 558049 for 10-15 minutes at 37oC, permeabilized BD Phosflow Perm Buffer II, Cat. No. 558052 on ice for 30 minutes, stained with Alexa Fluor 647 Mouse Anti-Human CD3 mAb UCHT1 Cat. No. 557706 , PerCP-Cy5.5 anti-human CD20 mAb H1 FB1 Cat. No. 558021 , and either PE Mouse anti-SLP-76 Cat. No. 560058, solid-line histograms or PE Mouse IgG2a, kappa Isotype control mAb MOPC-173 Cat. No. 558595, dashed-line histograms . The figures show lymphocyte subpopulations that were selected by their scatter profile and surface antigen expression. SLP-76 expression on CD3-positive CD20-negative T lymphocytes left panel , CD20-positive CD3-negative B lymphocytes center panel , and CD20-negative CD3-negative NK cells right panel are displayed. There was no detectable expression of SLP-76 in the monocyte/granulocyte population not shown . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Show More Analysis of SLP-76 in human peripheral blood lymphocytes. Human whole blood was lysed and fixed with 1X BD Phosflow Lyse/Fix Buffer Cat. No. 558049 for 10-15 minutes at 37oC, permeabilized BD Phosflow Perm Buffer II, Cat. No. 558052 on ice for 30 minutes, stained with Alexa Fluor 647 Mouse Anti-Human CD3 mAb UCHT1 Cat. No. 557706 , PerCP-Cy5.5 anti-human CD20 mAb H1 FB1 Cat. No. 558021 , and either PE Mouse anti-SLP-76 Cat. No. 560058, solid-line histograms or PE Mouse IgG2a, kappa Isotype control mAb MOPC-173 Cat. No. 558595, dashed-line histograms . The figures show lymphocyte subpopulations that were selected by their scatter profile and surface antigen expression. SLP-76 expression on CD3-positive CD20-negative T lymphocytes left panel , CD20-positive CD3-negative B lymphocytes center panel , and CD20-negative CD3-negative NK cells right panel are displayed. There was no detectable expression of SLP-76 in the monocyte/granulocyte population not shown . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Analysis of SLP-76 in human peripheral blood lymphocytes. Human whole blood was lysed and fixed with 1X BD Phosflow Lyse/Fix Buffer Cat. No. 558049 for 10-15 minutes at 37oC, permeabilized BD Phosflow Perm Buffer II, Cat. No. 558052 on ice for 30 minutes, stained with Alexa Fluor 647 Mouse Anti-Human CD3 mAb UCHT1 Cat. No. 557706 , PerCP-Cy5.5 anti-human CD20 mAb H1 FB1 Cat. No. 558021 , and either PE Mouse anti-SLP-76 Cat. No. 560058, solid-line histograms or PE Mouse IgG2a, kappa Isotype control mAb MOPC-173 Cat. No. 558595, dashed-line histograms . The figures show lymphocyte subpopulations that were selected by their scatter profile and surface antigen expression. SLP-76 expression on CD3-positive CD20-negative T lymphocytes left panel , CD20-positive CD3-negative B lymphocytes center panel , and CD20-negative CD3-negative NK cells right panel are displayed. There was no detectable expression of SLP-76 in the monocyte/granulocyte population not shown . Flow cytometry was performed on a BD FACSCalibur flow cytometry system.