BD PhosflowTM PE Mouse Anti-Stat1 (pY701)
612564 ·
BDB Bioscience · Cat: 612564
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bdbiosciences.com/en-us/products/reagents/flow-cytometry-rea...Description
Flow cytometric analysis of phospho-Stat1 pY701 . Human histiocytic lymphoma cells U937 were either left unstimulated unshaded or stimulated shaded with 1000 U/ml BD Pharmingen Recombinant Human IFNgamma Cat. No. 554617 for 15 minutes at 37C. Cells were fixed 10 min, 37C with BD Cytofix Fixation Buffer Cat. No. 554655 and then permeabilized on ice, 30 min with BD Phosflow Perm Buffer III Cat. No. 558050, 30 minutes on ice or overnight at -20C . Cells were then washed twice in BD Pharmingen Stain Buffer Cat. No. 554656/554567 and stained with PE Mouse Anti-Stat1 pY701 antibody Cat. No. 562069/612564 . The cells were analyzed on a BD FACSCalibur flow cytometer. Flow cytometric analysis of phospho-Stat1 pY701 . Human histiocytic lymphoma cells U937 were either left unstimulated unshaded or stimulated shaded with 1000 U/ml BD Pharmingen Recombinant Human IFNgamma Cat. No. 554617 for 15 minutes at 37C. Cells were fixed 10 min, 37C with BD Cytofix Fixation Buffer Cat. No. 554655 and then permeabilized on ice, 30 min with BD Phosflow Perm Buffer III Cat. No. 558050, 30 minutes on ice or overnight at -20C . Cells were then washed twice in BD Pharmingen Stain Buffer Cat. No. 554656/554567 and stained with PE Mouse Anti-Stat1 pY701 antibody Cat. No. 562069/612564 . The cells were analyzed on a BD FACSCalibur flow cytometer. Show More Flow cytometric analysis of phospho-Stat1 pY701 . Human histiocytic lymphoma cells U937 were either left unstimulated unshaded or stimulated shaded with 1000 U/ml BD Pharmingen Recombinant Human IFNgamma Cat. No. 554617 for 15 minutes at 37C. Cells were fixed 10 min, 37C with BD Cytofix Fixation Buffer Cat. No. 554655 and then permeabilized on ice, 30 min with BD Phosflow Perm Buffer III Cat. No. 558050, 30 minutes on ice or overnight at -20C . Cells were then washed twice in BD Pharmingen Stain Buffer Cat. No. 554656/554567 and stained with PE Mouse Anti-Stat1 pY701 antibody Cat. No. 562069/612564 . The cells were analyzed on a BD FACSCalibur flow cytometer.