BD PhosflowTM PE Mouse anti-Stat3 (pS727)

Description

Analysis of Stat3 pS727 in monocytes. Human peripheral blood mononuclear cells PBMC were either stimulated with 40 nM PMA Sigma, P8139 for 15 minutes shaded histogram or unstimulated open histogram . The cells were fixed BD Cytofix buffer, Cat. No. 554655 for 10 minutes, then permeabilized BD PhosFlow Perm Buffer III, Cat. No. 558050 on ice for at least 30 minutes, and then stained with PE Mouse anti-Stat3 pS727, Cat. No. 558557 . Monocytes were selected by scatter profile. Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Analysis of Stat3 pS727 in monocytes. Human peripheral blood mononuclear cells PBMC were either stimulated with 40 nM PMA Sigma, P8139 for 15 minutes shaded histogram or unstimulated open histogram . The cells were fixed BD Cytofix buffer, Cat. No. 554655 for 10 minutes, then permeabilized BD PhosFlow Perm Buffer III, Cat. No. 558050 on ice for at least 30 minutes, and then stained with PE Mouse anti-Stat3 pS727, Cat. No. 558557 . Monocytes were selected by scatter profile. Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Show More Analysis of Stat3 pS727 in monocytes. Human peripheral blood mononuclear cells PBMC were either stimulated with 40 nM PMA Sigma, P8139 for 15 minutes shaded histogram or unstimulated open histogram . The cells were fixed BD Cytofix buffer, Cat. No. 554655 for 10 minutes, then permeabilized BD PhosFlow Perm Buffer III, Cat. No. 558050 on ice for at least 30 minutes, and then stained with PE Mouse anti-Stat3 pS727, Cat. No. 558557 . Monocytes were selected by scatter profile. Flow cytometry was performed on a BD FACSCalibur flow cytometry system.