BD PhosflowTM PE Mouse Anti-Stat3 (pY705)
562072 ·
BDB Bioscience · Cat: 562072
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Flow cytometric analysis of Stat3 pY705 . Human whole blood was collected in the presence of heparin. Whole blood was either left unstimulated unshaded or stimulated shaded with recombinant human IL-6 MN 550071 at 100 ng/mL for 15 min at 37 C. Cells were lysed and fixed in a single step using BD Phosflow Lyse/Fix buffer MN 558049 for 10 min at 37 C. Cells were then permeabilized in BD Phosflow Perm Buffer III MN 558050 for 30 min on ice or overnight at -20 C. Cells were then washed twice in BD Pharmingen Stain Buffer MN 554656 and stained with PE mouse anti-Stat3 pY705 antibody for 30 min at room temperature. Samples were analyzed on a BD FACSCalibur instrument. Flow cytometric analysis of Stat3 pY705 . Human whole blood was collected in the presence of heparin. Whole blood was either left unstimulated unshaded or stimulated shaded with recombinant human IL-6 MN 550071 at 100 ng/mL for 15 min at 37 C. Cells were lysed and fixed in a single step using BD Phosflow Lyse/Fix buffer MN 558049 for 10 min at 37 C. Cells were then permeabilized in BD Phosflow Perm Buffer III MN 558050 for 30 min on ice or overnight at -20 C. Cells were then washed twice in BD Pharmingen Stain Buffer MN 554656 and stained with PE mouse anti-Stat3 pY705 antibody for 30 min at room temperature. Samples were analyzed on a BD FACSCalibur instrument. Show More Flow cytometric analysis of Stat3 pY705 . Human whole blood was collected in the presence of heparin. Whole blood was either left unstimulated unshaded or stimulated shaded with recombinant human IL-6 MN 550071 at 100 ng/mL for 15 min at 37 C. Cells were lysed and fixed in a single step using BD Phosflow Lyse/Fix buffer MN 558049 for 10 min at 37 C. Cells were then permeabilized in BD Phosflow Perm Buffer III MN 558050 for 30 min on ice or overnight at -20 C. Cells were then washed twice in BD Pharmingen Stain Buffer MN 554656 and stained with PE mouse anti-Stat3 pY705 antibody for 30 min at room temperature. Samples were analyzed on a BD FACSCalibur instrument.