BD PhosflowTM PE Mouse Anti-Stat3 (pY705)

Description

Flow cytometric analysis of Stat3 pY705 . Human whole blood was collected in the presence of heparin. Whole blood was either left unstimulated unshaded or stimulated shaded with recombinant human IL-6 Cat. No. 550071 at 100 ng/mL for 15 min at 37 C. Cells were lysed and fixed in a single step using BD Phosflow Lyse/Fix buffer Cat. No.558049 for 10 min at 37 C. Cells were then permeabilized in BD Phosflow Perm Buffer III Cat. No. 558050 for 30 min on ice or overnight at -20 C. Cells were then washed twice in BD Pharmingen Stain Buffer Cat. No. 554656 and stained with PE mouse anti-Stat3 pY705 antibody Cat. No. 612569 for 30 min at room temperature. Samples were analyzed on a BD FACSCalibur instrument. Flow cytometric analysis of Stat3 pY705 . Human whole blood was collected in the presence of heparin. Whole blood was either left unstimulated unshaded or stimulated shaded with recombinant human IL-6 Cat. No. 550071 at 100 ng/mL for 15 min at 37 C. Cells were lysed and fixed in a single step using BD Phosflow Lyse/Fix buffer Cat. No.558049 for 10 min at 37 C. Cells were then permeabilized in BD Phosflow Perm Buffer III Cat. No. 558050 for 30 min on ice or overnight at -20 C. Cells were then washed twice in BD Pharmingen Stain Buffer Cat. No. 554656 and stained with PE mouse anti-Stat3 pY705 antibody Cat. No. 612569 for 30 min at room temperature. Samples were analyzed on a BD FACSCalibur instrument. Show More Flow cytometric analysis of Stat3 pY705 . Human whole blood was collected in the presence of heparin. Whole blood was either left unstimulated unshaded or stimulated shaded with recombinant human IL-6 Cat. No. 550071 at 100 ng/mL for 15 min at 37 C. Cells were lysed and fixed in a single step using BD Phosflow Lyse/Fix buffer Cat. No.558049 for 10 min at 37 C. Cells were then permeabilized in BD Phosflow Perm Buffer III Cat. No. 558050 for 30 min on ice or overnight at -20 C. Cells were then washed twice in BD Pharmingen Stain Buffer Cat. No. 554656 and stained with PE mouse anti-Stat3 pY705 antibody Cat. No. 612569 for 30 min at room temperature. Samples were analyzed on a BD FACSCalibur instrument.