BD PhosflowTM PE Mouse Anti-Stat6 (pY641)

Description

Flow cytometric analysis of phospho-Stat6 Y641 . Human endothelial cells were starved overnight in DMEM containing 0.1 % FBS. The following day, cells were either left unstimulated unshaded or stimulated shaded with human IL-4 recombinant protein Cat. No. 554605 at 10 ng/ml for 60 minutes at 37C. Cells were fixed in BD Cytofix buffer 10 minutes at 37C and then permeabilized in BD Phosflow Perm Buffer III 30 minutes on ice or overnight at -20C . Cells were then washed twice in BD Pharmingen Stain Buffer Cat. No. 554656 , and stained with PE Mouse anti- Stat6 pY641 antibody. Flow cytometric analysis of phospho-Stat6 Y641 . Human endothelial cells were starved overnight in DMEM containing 0.1 % FBS. The following day, cells were either left unstimulated unshaded or stimulated shaded with human IL-4 recombinant protein Cat. No. 554605 at 10 ng/ml for 60 minutes at 37C. Cells were fixed in BD Cytofix buffer 10 minutes at 37C and then permeabilized in BD Phosflow Perm Buffer III 30 minutes on ice or overnight at -20C . Cells were then washed twice in BD Pharmingen Stain Buffer Cat. No. 554656 , and stained with PE Mouse anti- Stat6 pY641 antibody. Show More Flow cytometric analysis of phospho-Stat6 Y641 . Human endothelial cells were starved overnight in DMEM containing 0.1 % FBS. The following day, cells were either left unstimulated unshaded or stimulated shaded with human IL-4 recombinant protein Cat. No. 554605 at 10 ng/ml for 60 minutes at 37C. Cells were fixed in BD Cytofix buffer 10 minutes at 37C and then permeabilized in BD Phosflow Perm Buffer III 30 minutes on ice or overnight at -20C . Cells were then washed twice in BD Pharmingen Stain Buffer Cat. No. 554656 , and stained with PE Mouse anti- Stat6 pY641 antibody.