BD PhosflowTM PE Mouse anti-TBK1 (pS172)

Description

Analysis of TBK1 pS172 in transfected human epithelial cells. The 293 fetal kidney cell line was either co-transfected with TBK1 and IRF-7 expression vectors dashed histogram or un-transfected solid line . After 24 hours, the cells were fixed BD Cytofix buffer, Cat. No. 554655 for 10 minutes at 37 C, then permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for at least 30 minutes, and then stained with PE Mouse anti-TBK1 pS172 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Analysis of TBK1 pS172 in transfected human epithelial cells. The 293 fetal kidney cell line was either co-transfected with TBK1 and IRF-7 expression vectors dashed histogram or un-transfected solid line . After 24 hours, the cells were fixed BD Cytofix buffer, Cat. No. 554655 for 10 minutes at 37 C, then permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for at least 30 minutes, and then stained with PE Mouse anti-TBK1 pS172 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Show More Analysis of TBK1 pS172 in transfected human epithelial cells. The 293 fetal kidney cell line was either co-transfected with TBK1 and IRF-7 expression vectors dashed histogram or un-transfected solid line . After 24 hours, the cells were fixed BD Cytofix buffer, Cat. No. 554655 for 10 minutes at 37 C, then permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for at least 30 minutes, and then stained with PE Mouse anti-TBK1 pS172 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system.