BD PhosflowTM PerCP-CyTM5.5 Mouse Anti-Human CD20

Description

Analysis of CD20 cytoplasmic in human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were fixed with pre-warmed BD Cytofix buffer Cat. No. 554655 for 10 minutes at 37oC and permeabilized with BD Phosflow Perm Buffer II Cat. No. 558052 on ice for 30 minutes. The cells were then stained with either PerCP-Cy5.5 Mouse IgG2a, kappa, isotype control Cat. No. 558020, dashed line or PerCP-Cy5.5 Mouse Anti-Human CD20 cytoplasmic solid line . For data analysis, lymphocytes were selected by their scatter profile. CD20 cytoplasmic was not detected in the monocytes not shown . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Analysis of CD20 cytoplasmic in human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were fixed with pre-warmed BD Cytofix buffer Cat. No. 554655 for 10 minutes at 37oC and permeabilized with BD Phosflow Perm Buffer II Cat. No. 558052 on ice for 30 minutes. The cells were then stained with either PerCP-Cy5.5 Mouse IgG2a, kappa, isotype control Cat. No. 558020, dashed line or PerCP-Cy5.5 Mouse Anti-Human CD20 cytoplasmic solid line . For data analysis, lymphocytes were selected by their scatter profile. CD20 cytoplasmic was not detected in the monocytes not shown . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Analysis of CD20 cytoplasmic in human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were fixed with pre-warmed BD Cytofix buffer Cat. No. 554655 for 10 minutes at 37oC and permeabilized with BD Phosflow Perm Buffer II Cat. No. 558052 on ice for 30 minutes. The cells were then stained with either PerCP-Cy5.5 Mouse IgG2a, kappa, isotype control Cat. No. 558020, dashed line or PerCP-Cy5.5 Mouse Anti-Human CD20 cytoplasmic solid line . For data analysis, lymphocytes were selected by their scatter profile. CD20 cytoplasmic was not detected in the monocytes not shown . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Show More Analysis of CD20 cytoplasmic in human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were fixed with pre-warmed BD Cytofix buffer Cat. No. 554655 for 10 minutes at 37oC and permeabilized with BD Phosflow Perm Buffer II Cat. No. 558052 on ice for 30 minutes. The cells were then stained with either PerCP-Cy5.5 Mouse IgG2a, kappa, isotype control Cat. No. 558020, dashed line or PerCP-Cy5.5 Mouse Anti-Human CD20 cytoplasmic solid line . For data analysis, lymphocytes were selected by their scatter profile. CD20 cytoplasmic was not detected in the monocytes not shown . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Show More Analysis of CD20 cytoplasmic in human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were fixed with pre-warmed BD Cytofix buffer Cat. No. 554655 for 10 minutes at 37oC and permeabilized with BD Phosflow Perm Buffer II Cat. No. 558052 on ice for 30 minutes. The cells were then stained with either PerCP-Cy5.5 Mouse IgG2a, kappa, isotype control Cat. No. 558020, dashed line or PerCP-Cy5.5 Mouse Anti-Human CD20 cytoplasmic solid line . For data analysis, lymphocytes were selected by their scatter profile. CD20 cytoplasmic was not detected in the monocytes not shown . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Analysis of CD20 cytoplasmic in human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were fixed with pre-warmed BD Cytofix buffer Cat. No. 554655 for 10 minutes at 37oC and permeabilized with BD Phosflow Perm Buffer II Cat. No. 558052 on ice for 30 minutes. The cells were then stained with either PerCP-Cy5.5 Mouse IgG2a, kappa, isotype control Cat. No. 558020, dashed line or PerCP-Cy5.5 Mouse Anti-Human CD20 cytoplasmic solid line . For data analysis, lymphocytes were selected by their scatter profile. CD20 cytoplasmic was not detected in the monocytes not shown . Flow cytometry was performed on a BD FACSCalibur flow cytometry system.