BD PhosflowTM PerCP-CyTM5.5 Mouse Anti-Stat4 (pY693)

Description

Flow cytometric analysis of Stat4 pY693 expression in IFN-alpha treated human peripheral blood lymphocytes. Human whole blood was either stimulated with 40,000 U/ml of IFN-alpha for 15 minutes at 37oC solid line histogram or was left unstimulated dashed line histogram . The erythrocytes were lysed and the leukocytes were fixed with 1 BD Phosflow Lyse/Fix Buffer Cat. No. 558049 for 10-15 minutes at 37oC. The leukocytes were washed, permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for 30 minutes, washed and then stained with PerCP-Cy5.5 anti-Stat4 pY693 Cat. No.561217 . The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Flow cytometric analysis of Stat4 pY693 expression in IFN-alpha treated human peripheral blood lymphocytes. Human whole blood was either stimulated with 40,000 U/ml of IFN-alpha for 15 minutes at 37oC solid line histogram or was left unstimulated dashed line histogram . The erythrocytes were lysed and the leukocytes were fixed with 1 BD Phosflow Lyse/Fix Buffer Cat. No. 558049 for 10-15 minutes at 37oC. The leukocytes were washed, permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for 30 minutes, washed and then stained with PerCP-Cy5.5 anti-Stat4 pY693 Cat. No.561217 . The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Show More Flow cytometric analysis of Stat4 pY693 expression in IFN-alpha treated human peripheral blood lymphocytes. Human whole blood was either stimulated with 40,000 U/ml of IFN-alpha for 15 minutes at 37oC solid line histogram or was left unstimulated dashed line histogram . The erythrocytes were lysed and the leukocytes were fixed with 1 BD Phosflow Lyse/Fix Buffer Cat. No. 558049 for 10-15 minutes at 37oC. The leukocytes were washed, permeabilized BD Phosflow Perm Buffer III, Cat. No. 558050 on ice for 30 minutes, washed and then stained with PerCP-Cy5.5 anti-Stat4 pY693 Cat. No.561217 . The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System.