BD PhosflowTM R718 Mouse Anti-Akt (pS473)

Description

Flow cytometric analysis of Akt pS473 expression in human Jurkat cells. Cells from the human Jurkat acute T cell leukemia; ATCC TIB-152 cell line were either treated with 1 muM Wortmannin Life Technologies, Cat. No. PHZ1301 for 2 hours at 37C dashed line histogram or left untreated solid line histogram . The cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 for 10 minutes at 37C, permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 on ice for 30 minutes, and then stained with BD Phosflow R718 Mouse Anti-Akt pS473 antibody Cat. No. 567634 . The data demonstrate that the level of phosphorylation of Akt pS473 decreases when phosphatidylinositol 3-kinase activity is inhibited by the treatment of Jurkat cells with Wortmannin. The fluorescence histograms showing Akt pS473 expression were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis was performed on a BD LSR II Flow Cytometry System and FlowJo software. Flow cytometric analysis of Akt pS473 expression in human Jurkat cells. Cells from the human Jurkat acute T cell leukemia; ATCC TIB-152 cell line were either treated with 1 muM Wortmannin Life Technologies, Cat. No. PHZ1301 for 2 hours at 37C dashed line histogram or left untreated solid line histogram . The cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 for 10 minutes at 37C, permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 on ice for 30 minutes, and then stained with BD Phosflow R718 Mouse Anti-Akt pS473 antibody Cat. No. 567634 . The data demonstrate that the level of phosphorylation of Akt pS473 decreases when phosphatidylinositol 3-kinase activity is inhibited by the treatment of Jurkat cells with Wortmannin. The fluorescence histograms showing Akt pS473 expression were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis was performed on a BD LSR II Flow Cytometry System and FlowJo software. Show More Flow cytometric analysis of Akt pS473 expression in human Jurkat cells. Cells from the human Jurkat acute T cell leukemia; ATCC TIB-152 cell line were either treated with 1 muM Wortmannin Life Technologies, Cat. No. PHZ1301 for 2 hours at 37C dashed line histogram or left untreated solid line histogram . The cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 for 10 minutes at 37C, permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 on ice for 30 minutes, and then stained with BD Phosflow R718 Mouse Anti-Akt pS473 antibody Cat. No. 567634 . The data demonstrate that the level of phosphorylation of Akt pS473 decreases when phosphatidylinositol 3-kinase activity is inhibited by the treatment of Jurkat cells with Wortmannin. The fluorescence histograms showing Akt pS473 expression were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis was performed on a BD LSR II Flow Cytometry System and FlowJo software.