BD PhosflowTM R718 Mouse Anti-ERK1/2 (pT202/pY204)

Description

Flow cytometric analysis of Erk1/2 pT202/pY204 expression in stimulated human peripheral blood lymphocytes. Whole blood was either left untreated dashed line histogram or treated solid line histogram with 400 nM Phorbol 12-Myristate 13-Acetate PMA; Sigma, Cat. No. P8139 for 15 minutes at 37C. Erythrocytes were lysed and the leukocytes were fixed with 1X BD Phosflow Lyse/Fix Buffer Cat. No. 558049 for 10 minutes at 37C. The cells were then permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 on ice for 30 minutes and stained with BD Phosflow R718 Mouse Anti-Erk1/2 pT202/pY204 Cat. No. 570006/570087 . The fluorescence histograms showing ERK1/2 pT202/pY204 expression were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Flow cytometric analysis of Erk1/2 pT202/pY204 expression in stimulated human peripheral blood lymphocytes. Whole blood was either left untreated dashed line histogram or treated solid line histogram with 400 nM Phorbol 12-Myristate 13-Acetate PMA; Sigma, Cat. No. P8139 for 15 minutes at 37C. Erythrocytes were lysed and the leukocytes were fixed with 1X BD Phosflow Lyse/Fix Buffer Cat. No. 558049 for 10 minutes at 37C. The cells were then permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 on ice for 30 minutes and stained with BD Phosflow R718 Mouse Anti-Erk1/2 pT202/pY204 Cat. No. 570006/570087 . The fluorescence histograms showing ERK1/2 pT202/pY204 expression were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Show More Flow cytometric analysis of Erk1/2 pT202/pY204 expression in stimulated human peripheral blood lymphocytes. Whole blood was either left untreated dashed line histogram or treated solid line histogram with 400 nM Phorbol 12-Myristate 13-Acetate PMA; Sigma, Cat. No. P8139 for 15 minutes at 37C. Erythrocytes were lysed and the leukocytes were fixed with 1X BD Phosflow Lyse/Fix Buffer Cat. No. 558049 for 10 minutes at 37C. The cells were then permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 on ice for 30 minutes and stained with BD Phosflow R718 Mouse Anti-Erk1/2 pT202/pY204 Cat. No. 570006/570087 . The fluorescence histograms showing ERK1/2 pT202/pY204 expression were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software.