BD PhosflowTM R718 Mouse Anti-p38 MAPK (pT180/pY182)

Description

Flow cytometric analysis of p38 MAPK pT180/pY182 expression in monocytes. Human peripheral blood mononuclear cells were either untreated dashed line histogram or treated solid line histogram by culture with 40 muM Anisomycin for 25 minutes at 37C. The cells were fixed 10 min, 37C with BD Cytofix Fixation Buffer Cat. No. 554655 and then permeabilized on ice, 30 min with BD Phosflow Perm Buffer III, Cat. No. 558050. Cells were washed twice in BD Pharmingen Stain Buffer FBS Cat. No. 554656 and then stained with BD Phosflow R718 Mouse Anti-p38 MAPK pT180/pY182 antibody Cat. No. 563569 . The fluorescence histograms showing the expressed levels of p38 MAPK pT180/pY182 was derived from gated events with the forward and side light-scatter characteristics of intact monocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Flow cytometric analysis of p38 MAPK pT180/pY182 expression in monocytes. Human peripheral blood mononuclear cells were either untreated dashed line histogram or treated solid line histogram by culture with 40 muM Anisomycin for 25 minutes at 37C. The cells were fixed 10 min, 37C with BD Cytofix Fixation Buffer Cat. No. 554655 and then permeabilized on ice, 30 min with BD Phosflow Perm Buffer III, Cat. No. 558050. Cells were washed twice in BD Pharmingen Stain Buffer FBS Cat. No. 554656 and then stained with BD Phosflow R718 Mouse Anti-p38 MAPK pT180/pY182 antibody Cat. No. 563569 . The fluorescence histograms showing the expressed levels of p38 MAPK pT180/pY182 was derived from gated events with the forward and side light-scatter characteristics of intact monocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Flow cytometric analysis of p38 MAPK pT180/pY182 expression in monocytes. Human peripheral blood mononuclear cells were either untreated dashed line histogram or treated solid line histogram by culture with 40 muM Anisomycin for 25 minutes at 37C. The cells were fixed 10 min, 37C with BD Cytofix Fixation Buffer Cat. No. 554655 and then permeabilized on ice, 30 min with BD Phosflow Perm Buffer III, Cat. No. 558050. Cells were washed twice in BD Pharmingen Stain Buffer FBS Cat. No. 554656 and then stained with BD Phosflow R718 Mouse Anti-p38 MAPK pT180/pY182 antibody Cat. No. 563569 . The fluorescence histograms showing the expressed levels of p38 MAPK pT180/pY182 was derived from gated events with the forward and side light-scatter characteristics of intact monocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software.