BD PhosflowTM RB613 Mouse Anti-Stat3 (pY705)

Description

Analysis of Stat3 pY705 expression in Human peripheral blood lymphocytes. Human whole blood was either left Unstimulated dashed line histogram or Stimulated solid line histogram with 100 ng/ml of BD Pharmingen Recombinant Human IL-6 protein Cat. No. 550071 for 15 minutes at 37C. The leukocytes were fixed and the erythrocytes were lysed in a single step by mixing and incubation with pre-warmed BD Phosflow Lyse/Fix Buffer Cat. No. 558049 for 10 min at 37C. The leukocytes were washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , pelleted by centrifugation and then resuspended and left in BD Phosflow Perm Buffer III Cat. No. 558052; chilled to -20C before use for 30 minutes on ice. The cells were washed twice with BD Pharmingen Stain Buffer FBS and then stained with BD Phosflow RB613 Mouse Anti-Stat3 pY705 antibody Cat. No. 571242/571306 . The fluorescence histograms showing Stat3 pY705 expression were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo Software. Analysis of Stat3 pY705 expression in Human peripheral blood lymphocytes. Human whole blood was either left Unstimulated dashed line histogram or Stimulated solid line histogram with 100 ng/ml of BD Pharmingen Recombinant Human IL-6 protein Cat. No. 550071 for 15 minutes at 37C. The leukocytes were fixed and the erythrocytes were lysed in a single step by mixing and incubation with pre-warmed BD Phosflow Lyse/Fix Buffer Cat. No. 558049 for 10 min at 37C. The leukocytes were washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , pelleted by centrifugation and then resuspended and left in BD Phosflow Perm Buffer III Cat. No. 558052; chilled to -20C before use for 30 minutes on ice. The cells were washed twice with BD Pharmingen Stain Buffer FBS and then stained with BD Phosflow RB613 Mouse Anti-Stat3 pY705 antibody Cat. No. 571242/571306 . The fluorescence histograms showing Stat3 pY705 expression were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo Software. Show More Analysis of Stat3 pY705 expression in Human peripheral blood lymphocytes. Human whole blood was either left Unstimulated dashed line histogram or Stimulated solid line histogram with 100 ng/ml of BD Pharmingen Recombinant Human IL-6 protein Cat. No. 550071 for 15 minutes at 37C. The leukocytes were fixed and the erythrocytes were lysed in a single step by mixing and incubation with pre-warmed BD Phosflow Lyse/Fix Buffer Cat. No. 558049 for 10 min at 37C. The leukocytes were washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , pelleted by centrifugation and then resuspended and left in BD Phosflow Perm Buffer III Cat. No. 558052; chilled to -20C before use for 30 minutes on ice. The cells were washed twice with BD Pharmingen Stain Buffer FBS and then stained with BD Phosflow RB613 Mouse Anti-Stat3 pY705 antibody Cat. No. 571242/571306 . The fluorescence histograms showing Stat3 pY705 expression were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo Software.