BD PhosflowTM RB705 Mouse Anti-Stat3 (pY705)

Description

Analysis of Stat3 pY705 expressed in Human peripheral blood lymphocytes. Human whole blood was either left unstimulated dashed line histogram or stimulated solid line histogram with 100 ng/ml BD Pharmingen Recombinant Human IL-6 protein Cat. No. 550071 for 15 minutes at 37C. Erythrocytes were lysed and the leukocytes were fixed in a single step using BD Phosflow Lyse/Fix Buffer Cat. No. 558049 for 10 min at 37 C. Cells were then permeabilized in BD Phosflow Perm Buffer III Cat. No. 558050 on ice for 30 minutes, washed twice with 5-10 mL of BD Pharmingen Stain Buffer FBS and then stained with BD Phosflow RB705 Mouse Anti-Stat3 pY705 antibody Cat. No. 570575/570663 . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Analysis of Stat3 pY705 expressed in Human peripheral blood lymphocytes. Human whole blood was either left unstimulated dashed line histogram or stimulated solid line histogram with 100 ng/ml BD Pharmingen Recombinant Human IL-6 protein Cat. No. 550071 for 15 minutes at 37C. Erythrocytes were lysed and the leukocytes were fixed in a single step using BD Phosflow Lyse/Fix Buffer Cat. No. 558049 for 10 min at 37 C. Cells were then permeabilized in BD Phosflow Perm Buffer III Cat. No. 558050 on ice for 30 minutes, washed twice with 5-10 mL of BD Pharmingen Stain Buffer FBS and then stained with BD Phosflow RB705 Mouse Anti-Stat3 pY705 antibody Cat. No. 570575/570663 . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Show More Analysis of Stat3 pY705 expressed in Human peripheral blood lymphocytes. Human whole blood was either left unstimulated dashed line histogram or stimulated solid line histogram with 100 ng/ml BD Pharmingen Recombinant Human IL-6 protein Cat. No. 550071 for 15 minutes at 37C. Erythrocytes were lysed and the leukocytes were fixed in a single step using BD Phosflow Lyse/Fix Buffer Cat. No. 558049 for 10 min at 37 C. Cells were then permeabilized in BD Phosflow Perm Buffer III Cat. No. 558050 on ice for 30 minutes, washed twice with 5-10 mL of BD Pharmingen Stain Buffer FBS and then stained with BD Phosflow RB705 Mouse Anti-Stat3 pY705 antibody Cat. No. 570575/570663 . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software.